Detailed information of TA system TA156924

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2884417..2886005
• Replicon: chromosome
• Accession: NZ_CP053594
• Organism: Escherichia coli strain T7Express_Crystal

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: S1ELM3
• Locus tag: HO402_RS13775
• Protein ID: WP_001125439.1
• Coordinates: 2884683..2886005 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: HO402_RS13770
• Protein ID: WP_001301023.1
• Coordinates: 2884417..2884683 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HO402_RS13745	2879592..2879882	-	291	WP_000558527.1	(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione isomerase	-
HO402_RS13750	2879906..2880781	-	876	WP_000774188.1	3-hydroxy-5-phosphonooxypentane-2,4-dione thiolase	-
HO402_RS13755	2880808..2881830	-	1023	WP_000172488.1	autoinducer 2 ABC transporter substrate-binding protein LsrB	-
HO402_RS13760	2881842..2882618	-	777	Protein_2672	histidine kinase	-
HO402_RS13765	2882614..2884194	+	1581	Protein_2673	autotransporter outer membrane beta-barrel domain-containing protein	-
HO402_RS13770	2884417..2884683	+	267	WP_001301023.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
HO402_RS13775	2884683..2886005	+	1323	WP_001125439.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
HO402_RS13780	2886377..2886859	+	483	WP_000931227.1	fimbrial protein	-
HO402_RS13785	2887220..2887930	+	711	Protein_2677	fimbria/pilus periplasmic chaperone	-
HO402_RS13790	2887972..2888322	+	351	WP_001354079.1	usher protein	-
HO402_RS13795	2888277..2889410	+	1134	WP_001354080.1	fimbrial biogenesis outer membrane usher protein	-
HO402_RS13800	2889474..2890622	+	1149	WP_000017447.1	fimbrial biogenesis outer membrane usher protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49275.82 Da
• Isoelectric Point: 8.2482

>T156924 WP_001125439.1 NZ_CP053594:2884683-2886005 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK

Nucleotide

• Length: 1323 bp

>T156924 NZ_CP069073:1975934-1976182 [Mycobacterium tuberculosis]
ATGGTGATCGATACCTCTGCGCTGGTCGCGATGCTCAACGATGAACCCGAGGCGCAACGGTTCGAGATAGCCGTGGCAGC
AGACCACGTTTGGCTGATGTCGACGGCGTCATATCCGGAGATGGCGACCGTGATCGAAACACGCTTCGGGGAACCGGGGG
GACGTGAACCCAAGGTCAGCGGCCAGCCTCTCCTCTATAAGGGTGACGATTTCGCATGTATCGATATTCGCGCGGTTCTC
GCCGGCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10016.38 Da
• Isoelectric Point: 6.2267

>AT156924 WP_001301023.1 NZ_CP053594:2884417-2884683 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDAKNASPES
TEQQNLEW

Nucleotide

• Length: 267 bp

>AT156924 NZ_CP069073:1975722-1975934 [Mycobacterium tuberculosis]
ATGGAATTGGCGGCTCGAATGGGCGAGACTTTGACACAAGCGGTCGTAGTTGCAGTGCGGGAGCAACTGGCCCGCCGGAC
CGGGCGCACCAGATCCATTTCGCTACGCGAGGAGTTGGCCGCCATTGGCCGGCGCTGCGCGGCCTTACCGGTGCTCGACA
CCCGAGCCGCGGACACGATTCTCGGCTACGACGAGCGCGGGTTGCCCGCCTGA

Structures

Toxin

• PDB: 5K98
• PDB: 3TPV
• PDB: 3DNV
• PDB: 3TPD
• PDB: 3HZI
• PDB: 3FBR
• PDB: 4YG7
• PDB: 3TPE
• PDB: 2WIU
• PDB: 3DNT
• PDB: 3TPB
• PDB: 3TPT
• PDB: 3DNU