Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2971330..2971968 Replicon chromosome
Accession NZ_CP053592
Organism Escherichia coli strain NEBExpress_Iq

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag HO403_RS14160 Protein ID WP_000813794.1
Coordinates 2971792..2971968 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag HO403_RS14155 Protein ID WP_001270286.1
Coordinates 2971330..2971746 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HO403_RS14135 2966482..2967423 - 942 WP_001251313.1 ABC transporter permease -
HO403_RS14140 2967424..2968437 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
HO403_RS14145 2968455..2969600 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
HO403_RS14150 2969845..2971251 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
HO403_RS14155 2971330..2971746 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
HO403_RS14160 2971792..2971968 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
HO403_RS14165 2972190..2972420 + 231 WP_000494244.1 YncJ family protein -
HO403_RS14170 2972512..2974473 - 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
HO403_RS14175 2974546..2975082 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
HO403_RS14180 2975135..2976349 + 1215 WP_001326689.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T156905 WP_000813794.1 NZ_CP053592:c2971968-2971792 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T156905 NZ_CP069073:709029-709430 [Mycobacterium tuberculosis]
GTGATCGTCGACACGTCGGCGATCATCGCGATTCTGCGCGACGAGGACGACGCCGCGGCCTACGCCGACGCGCTCGCCAA
CGCCGATGTCCGCAGACTGTCTGCGGCCAGCTACCTGGAATGCGGGATAGTCCTTGACTCCCAGCGTGATCCGGTCATCA
GCAGAGCACTGGATGAACTTATCGAAGAAGCCGAGTTCGTCGTCGAGCCGGTAACCGAGCGCCAGGCCCGCCTGGCCCGA
GCGGCCTACGCGGATTTCGGCAGAGGCAGCGGCCACCCCGCGGGCTTGAATTTCGGCGACTGCCTGTCCTATGCACTGGC
GATCGATCGACGTGAGCCGCTGCTGTGGAAGGGCAACGACTTTGGGCACACCGGCGTCCAAAGGGCACTGGATCGGCGGT
GA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT156905 WP_001270286.1 NZ_CP053592:c2971746-2971330 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT156905 NZ_CP069073:708787-709032 [Mycobacterium tuberculosis]
ATGGCGTTGAACATCAAAGATCCGTCGGTTCACCAGGCGGTCAAGCAGATCGCGAAAATCACCGGCGAATCTCAGGCTCG
GGCGGTGGCGACCGCGGTGAACGAGCGTCTGGCCAGACTGCGCAGCGACGATCTCGCCGCCCGGCTCTTGGCTATCGGCC
ACAAGACCGCGAGCAGGATGAGCCCGGAAGCAAAGCGCCTCGACCACGATGCTCTGCTGTATGACGAGCGAGGGCTGCCG
GCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References