Detailed information of TA system TA156884

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 167706..168349
• Replicon: plasmid unnamed1
• Accession: NZ_CP053574
• Organism: Citrobacter sp. TSA-1

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: HF677_RS24190
• Protein ID: WP_095715190.1
• Coordinates: 167933..168349 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: HF677_RS24185
• Protein ID: WP_095715191.1
• Coordinates: 167706..167936 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF677_RS24145	163078..164049	-	972	WP_095715521.1	ParB/RepB/Spo0J family partition protein	-
HF677_RS24150	164046..165251	-	1206	WP_095715520.1	AAA family ATPase	-
HF677_RS24155	165502..165879	-	378	Protein_175	tyrosine-type recombinase/integrase	-
HF677_RS24160	165974..166144	-	171	WP_168726205.1	tyrosine-type recombinase/integrase	-
HF677_RS24165	166344..166595	+	252	WP_095715194.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
HF677_RS24170	166592..166879	+	288	WP_095715193.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HF677_RS24175	166885..167250	+	366	WP_141244065.1	hypothetical protein	-
HF677_RS24180	167247..167555	+	309	WP_095715192.1	hypothetical protein	-
HF677_RS24185	167706..167936	+	231	WP_095715191.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HF677_RS24190	167933..168349	+	417	WP_095715190.1	type II toxin-antitoxin system VapC family toxin	Toxin
HF677_RS24195	168382..169125	+	744	WP_095715189.1	hypothetical protein	-
HF677_RS24200	169361..169888	-	528	WP_095715188.1	GNAT family N-acetyltransferase	-
HF677_RS24205	169892..170161	-	270	WP_080172499.1	DUF1778 domain-containing protein	-
HF677_RS24210	170672..170878	-	207	WP_095715186.1	DUF4222 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	vipA/tssB / vipB/tssC / hcp/tssD / clpV/tssH / pefD / rck / pefD	1..171562	171562

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15152.52 Da
• Isoelectric Point: 7.2452

>T156884 WP_095715190.1 NZ_CP053574:167933-168349 [Citrobacter sp. TSA-1]
VNKTYMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASQRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T156884 NZ_CP069072:c3069765-3069349 [Mycobacterium tuberculosis]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8609.65 Da
• Isoelectric Point: 4.4563

>AT156884 WP_095715191.1 NZ_CP053574:167706-167936 [Citrobacter sp. TSA-1]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFASQEKADADFMAEREDVVTDEGRVTL

Nucleotide

• Length: 231 bp

>AT156884 NZ_CP069072:c3070028-3069762 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA