Detailed information of TA system TA156881

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 69922..70661
• Replicon: plasmid unnamed1
• Accession: NZ_CP053574
• Organism: Citrobacter sp. TSA-1

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: HF677_RS23620
• Protein ID: WP_095715504.1
• Coordinates: 69922..70407 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: HF677_RS23625
• Protein ID: WP_095715503.1
• Coordinates: 70395..70661 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF677_RS23595	65156..66571	+	1416	WP_095715506.1	AAA family ATPase	-
HF677_RS23600	66702..67463	-	762	WP_080950113.1	hypothetical protein	-
HF677_RS23605	67920..68840	+	921	WP_095715505.1	hypothetical protein	-
HF677_RS23610	68936..69055	-	120	Protein_66	IS3 family transposase	-
HF677_RS23615	69175..69462	-	288	WP_172864464.1	hypothetical protein	-
HF677_RS23620	69922..70407	-	486	WP_095715504.1	GNAT family N-acetyltransferase	Toxin
HF677_RS23625	70395..70661	-	267	WP_095715503.1	DUF1778 domain-containing protein	Antitoxin
HF677_RS23630	70979..71185	-	207	Protein_70	IS3 family transposase	-
HF677_RS23635	71749..72306	+	558	WP_095715502.1	Ail/Lom family outer membrane beta-barrel protein	-
HF677_RS23640	72341..72547	+	207	Protein_72	IS21 family transposase	-
HF677_RS23645	72540..73037	-	498	WP_168726214.1	hypothetical protein	-
HF677_RS23650	73118..74650	+	1533	Protein_74	IS3 family transposase	-
HF677_RS23655	74704..75198	-	495	Protein_75	transposase	-
HF677_RS23660	75399..75602	-	204	WP_141244120.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	vipA/tssB / vipB/tssC / hcp/tssD / clpV/tssH / pefD / rck / pefD	1..171562	171562

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (35-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17564.29 Da
• Isoelectric Point: 9.8712

>T156881 WP_095715504.1 NZ_CP053574:c70407-69922 [Citrobacter sp. TSA-1]
VGRVTAPEPLSSSHQLAEFVSGEGVLDDWLKQRGLKNQALGAARTFVVCKTDTKQVVGFYSLATGSVNHVEATGSLRRNM
PDPIPVIILARLAVDVSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYLHHGFKASQTKERTLFLKLP
Q

Nucleotide

• Length: 486 bp

>T156881 NZ_CP069072:c2870507-2870112 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10107.48 Da
• Isoelectric Point: 4.3837

>AT156881 WP_095715503.1 NZ_CP053574:c70661-70395 [Citrobacter sp. TSA-1]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDAAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT156881 NZ_CP069072:c2870749-2870504 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA