Detailed information of TA system TA156879

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4278006..4278709
• Replicon: chromosome
• Accession: NZ_CP053573
• Organism: Citrobacter sp. TSA-1

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: HF677_RS20375
• Protein ID: WP_095715650.1
• Coordinates: 4278006..4278347 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: HF677_RS20380
• Protein ID: WP_063308407.1
• Coordinates: 4278368..4278709 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF677_RS20345	4273730..4274092	+	363	WP_095715652.1	endoribonuclease SymE	-
HF677_RS20350	4274155..4274640	-	486	WP_016151545.1	type VI secretion system tube protein Hcp	-
HF677_RS20355	4274659..4274985	-	327	WP_016155588.1	DUF1493 family protein	-
HF677_RS20360	4274979..4275428	-	450	WP_003830103.1	hypothetical protein	-
HF677_RS20365	4275687..4276559	+	873	WP_095715651.1	HNH endonuclease	-
HF677_RS20370	4276740..4277747	-	1008	WP_001757896.1	restriction endonuclease	-
HF677_RS20375	4278006..4278347	-	342	WP_095715650.1	TA system toxin CbtA family protein	Toxin
HF677_RS20380	4278368..4278709	-	342	WP_063308407.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HF677_RS20385	4278720..4279262	-	543	WP_095715649.1	DNA repair protein RadC	-
HF677_RS20390	4279275..4279718	-	444	WP_063308409.1	antirestriction protein	-
HF677_RS20395	4279749..4280570	-	822	WP_095715648.1	DUF945 domain-containing protein	-
HF677_RS20400	4280691..4281164	-	474	WP_032689716.1	hypothetical protein	-
HF677_RS20405	4281236..4281688	-	453	WP_052415655.1	hypothetical protein	-
HF677_RS20410	4281724..4282440	-	717	WP_032689717.1	WYL domain-containing protein	-
HF677_RS20415	4282684..4283559	-	876	WP_048261928.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4274979..4303265	28286

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (13-111)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12782.75 Da
• Isoelectric Point: 8.0324

>T156879 WP_095715650.1 NZ_CP053573:c4278347-4278006 [Citrobacter sp. TSA-1]
MKTLPATTPQAATLCLSPVTVWQMLLARLLEQHYGLTLNDTPFSDETVIQEHIDAGISLADAVNFLMEKYGLVRIDRRGF
NWEEQSPYLRAVDILRARQATGLLKRNRISAAR

Nucleotide

• Length: 342 bp

>T156879 NZ_CP069072:2868539-2868952 [Mycobacterium tuberculosis]
ATGGTGTTCTGCGTCGACACCAGCGCGTGGCATCACGCGGCGCGGCCGGAAGTTGCGCGCCGATGGTTGGCGGCCTTGTC
CGCGGACCAGATCGGCATCTGCGACCACGTGCGGTTGGAGATCCTGTACTCGGCGAACTCCGCTACCGACTACGACGCGC
TCGCCGACGAACTCGACGGCTTGGCCCGTATACCAGTCGGTGCCGAAACCTTTACGCGCGCATGCCAAGTCCAGCGTGAG
CTTGCCCACGTCGCCGGTCTGCATCACCGCAGCGTGAAGATCGCCGATCTTGTCATCGCCGCGGCGGCCGAACTTTCAGG
CACCATCGTGTGGCATTACGACGAGAACTATGACCGGGTCGCCGCCATCACCGGCCAACCTACGGAGTGGATCGTGCCGC
GCGGGACCCTTTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12722.34 Da
• Isoelectric Point: 6.4739

>AT156879 WP_063308407.1 NZ_CP053573:c4278709-4278368 [Citrobacter sp. TSA-1]
MKSTDSENDSFLQWGLNRNVTPCFGARLVQEGNRLHYLADRASITGKFSEAECLKLDVAFPHFISQMESMLTTGEMNPRH
AHCVTLYHNGFTCEADTLGSCGYVYIAVYPTHR

Nucleotide

• Length: 342 bp

>AT156879 NZ_CP069072:2868991-2869248 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA