Detailed information of TA system TA156855

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 9592..10235
• Replicon: plasmid pMY490-2
• Accession: NZ_CP053570
• Organism: Enterobacter cloacae strain MY490

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q84A06
• Locus tag: HPB40_RS26810
• Protein ID: WP_000754566.1
• Coordinates: 9819..10235 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: HPB40_RS26805
• Protein ID: WP_001261276.1
• Coordinates: 9592..9822 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPB40_RS26770 (HPB40_26700)	4711..4980	+	270	WP_000339857.1	hypothetical protein	-
HPB40_RS26775 (HPB40_26705)	5157..6023	-	867	WP_004118283.1	replication initiation protein	-
HPB40_RS26780 (HPB40_26710)	6553..6657	+	105	WP_032409716.1	hypothetical protein	-
HPB40_RS26785 (HPB40_26715)	6786..7043	+	258	WP_000764642.1	hypothetical protein	-
HPB40_RS26790 (HPB40_26720)	7101..7877	-	777	WP_000015958.1	site-specific integrase	-
HPB40_RS26795 (HPB40_26725)	7874..8617	-	744	WP_000129823.1	hypothetical protein	-
HPB40_RS26800 (HPB40_26730)	8668..9018	-	351	WP_000493378.1	hypothetical protein	-
HPB40_RS26805 (HPB40_26735)	9592..9822	+	231	WP_001261276.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
HPB40_RS26810 (HPB40_26740)	9819..10235	+	417	WP_000754566.1	type II toxin-antitoxin system VapC family toxin	Toxin
HPB40_RS26815 (HPB40_26745)	10439..11295	+	857	Protein_14	IS3-like element ISEc15 family transposase	-
HPB40_RS26820 (HPB40_26750)	11577..12653	+	1077	WP_000227969.1	IS110 family transposase	-
HPB40_RS26825 (HPB40_26755)	12661..12774	-	114	Protein_16	Tn3 family transposase	-
HPB40_RS26830 (HPB40_26765)	13301..14161	-	861	WP_000027057.1	broad-spectrum class A beta-lactamase TEM-1	-
HPB40_RS26835 (HPB40_26770)	14344..14901	-	558	WP_001235713.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaTEM-1B / qnrS1 / sul1 / qacE / aadA2 / dfrA12 / floR / sul2 / tet(A) / aph(3'')-Ib / aph(6)-Id	-	1..55609	55609

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15006.52 Da
• Isoelectric Point: 9.2957

>T156855 WP_000754566.1 NZ_CP053570:9819-10235 [Enterobacter cloacae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T156855 NZ_CP069072:1018199-1018633 [Mycobacterium tuberculosis]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT156855 WP_001261276.1 NZ_CP053570:9592-9822 [Enterobacter cloacae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT156855 NZ_CP069072:1018014-1018193 [Mycobacterium tuberculosis]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCATGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Structures

Toxin

• AlphaFold DB: A0A387K1G3

Antitoxin

• AlphaFold DB: A0A387K023