Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | hipBA/HipA-HipB |
| Location | 14073..15696 | Replicon | plasmid pMY490-mcr-9 |
| Accession | NZ_CP053569 | ||
| Organism | Enterobacter cloacae strain MY490 | ||
Toxin (Protein)
| Gene name | hipA | Uniprot ID | C1IUI3 |
| Locus tag | HPB40_RS24800 | Protein ID | WP_000088044.1 |
| Coordinates | 14371..15696 (+) | Length | 442 a.a. |
Antitoxin (Protein)
| Gene name | hipB | Uniprot ID | A0A6M3PBR7 |
| Locus tag | HPB40_RS24795 | Protein ID | WP_012477377.1 |
| Coordinates | 14073..14366 (+) | Length | 98 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| HPB40_RS24775 (HPB40_24720) | 9872..10264 | + | 393 | WP_000341066.1 | hypothetical protein | - |
| HPB40_RS24780 (HPB40_24725) | 10923..11579 | + | 657 | WP_000462754.1 | trypsin-like serine protease | - |
| HPB40_RS24785 (HPB40_24730) | 11782..12279 | + | 498 | WP_000340139.1 | membrane protein | - |
| HPB40_RS24790 (HPB40_24735) | 12284..13672 | + | 1389 | WP_000083579.1 | hypothetical protein | - |
| HPB40_RS24795 (HPB40_24740) | 14073..14366 | + | 294 | WP_012477377.1 | helix-turn-helix transcriptional regulator | Antitoxin |
| HPB40_RS24800 (HPB40_24745) | 14371..15696 | + | 1326 | WP_000088044.1 | type II toxin-antitoxin system HipA family toxin | Toxin |
| HPB40_RS24805 (HPB40_24750) | 15757..15963 | + | 207 | WP_000134171.1 | hypothetical protein | - |
| HPB40_RS24810 (HPB40_24755) | 16064..16474 | + | 411 | WP_000985909.1 | hypothetical protein | - |
| HPB40_RS24815 (HPB40_24760) | 16529..16948 | + | 420 | WP_233002139.1 | hypothetical protein | - |
| HPB40_RS24820 (HPB40_24765) | 17130..18132 | - | 1003 | Protein_21 | IS110-like element IS4321 family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | ant(2'')-Ia / catB8 / blaOXA-10 / ant(3'')-Ia / qacE / sul1 / aph(3')-Ia / mph(A) / dfrA12 / aadA1 / formA / mcr-9 | - | 1..381054 | 381054 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 442 a.a. Molecular weight: 50333.32 Da Isoelectric Point: 6.5044
>T156854 WP_000088044.1 NZ_CP053569:14371-15696 [Enterobacter cloacae]
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK
MSRKQQRLAIWMNGIKVGYWEKSKGVDSLQYLPDWSADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMRSSVENEWLCSVLLEQYGLPVARTQIAHFEDQKALVVERF
DRKWSGNGQWIIRLPQEDMCQALGISPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIVFWMMAATDGHAKNFS
ISIGPQGRYHLTPLYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK
Download Length: 1326 bp
>T156854 NZ_CP069072:844524-844952 [Mycobacterium tuberculosis]
ATGTTCCTTCTCGACGCCAACGTGCTGCTGGCTGCACACCGCGGTGACCACCCGAATCACCGAACCGTCCGCCCCTGGTT
CGATCGACTGCTCGCGGCTGACGACCCCTTCACAGTGCCGAACCTGGTATGGGCGTCGTTCCTCCGGCTGGCAACGAATC
GACGCATCTTCGAGATTCCGTCACCGCGAGCAGAGGCATTCGCATTCGTCGAAGCCGTCACCGCCCAGCCCCATCACCTT
CCGACGAACCCCGGTCCCAGACACCTCATGCTGCTGCGAAAACTCTGCGACGAGGCCGACGCATCGGGCGACTTGATACC
TGACGCGGTACTCGCGGCCATAGCAGTGGGGCATCACTGCGCCGTGGTGAGCCTGGACAGGGATTTCGCCCGGTTTGCCT
CGGTGCGCCACATTCGCCCGCCGCTCTAG
ATGTTCCTTCTCGACGCCAACGTGCTGCTGGCTGCACACCGCGGTGACCACCCGAATCACCGAACCGTCCGCCCCTGGTT
CGATCGACTGCTCGCGGCTGACGACCCCTTCACAGTGCCGAACCTGGTATGGGCGTCGTTCCTCCGGCTGGCAACGAATC
GACGCATCTTCGAGATTCCGTCACCGCGAGCAGAGGCATTCGCATTCGTCGAAGCCGTCACCGCCCAGCCCCATCACCTT
CCGACGAACCCCGGTCCCAGACACCTCATGCTGCTGCGAAAACTCTGCGACGAGGCCGACGCATCGGGCGACTTGATACC
TGACGCGGTACTCGCGGCCATAGCAGTGGGGCATCACTGCGCCGTGGTGAGCCTGGACAGGGATTTCGCCCGGTTTGCCT
CGGTGCGCCACATTCGCCCGCCGCTCTAG
Antitoxin
Download Length: 98 a.a. Molecular weight: 10860.30 Da Isoelectric Point: 10.3134
>AT156854 WP_012477377.1 NZ_CP053569:14073-14366 [Enterobacter cloacae]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSTTASSEGK
QTEEMLKSNSPARQEKW
Download Length: 294 bp
>AT156854 NZ_CP069072:844243-844500 [Mycobacterium tuberculosis]
ATGCGCACCACGGTGTCAATCTCCGATGAAATACTCGCTGCCGCCAAACGCCGGGCCCGCGAGCGTGGTCAATCGCTGGG
CGCTGTGATCGAGGACGCCCTTCGGCGGGAGTTCGCCGCCGCCCACGTCGGCGGCGCCCGCCCGACCGTCCCGGTTTTCG
ACGGCGGCACCGGTCCGCGGCGAGGCATCGACCTGACCTCGAATAGAGCGTTGTCCGAAGTGCTCGACGAGGGCCTGGAA
CTGAACTCCCGGAAGTAA
ATGCGCACCACGGTGTCAATCTCCGATGAAATACTCGCTGCCGCCAAACGCCGGGCCCGCGAGCGTGGTCAATCGCTGGG
CGCTGTGATCGAGGACGCCCTTCGGCGGGAGTTCGCCGCCGCCCACGTCGGCGGCGCCCGCCCGACCGTCCCGGTTTTCG
ACGGCGGCACCGGTCCGCGGCGAGGCATCGACCTGACCTCGAATAGAGCGTTGTCCGAAGTGCTCGACGAGGGCCTGGAA
CTGAACTCCCGGAAGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2S1XXW1 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A6M3PBR7 |