156840

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) KacT-ataR/DUF1778(antitoxin)
Location 1421647..1422441 Replicon chromosome
Accession NZ_CP053568
Organism Enterobacter cloacae strain MY490

Toxin (Protein)


Gene name KacT Uniprot ID A0A7V7JFS7
Locus tag HPB40_RS06860 Protein ID WP_013095427.1
Coordinates 1421647..1422168 (-) Length 174 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID A0A5E1AP76
Locus tag HPB40_RS06865 Protein ID WP_013095428.1
Coordinates 1422172..1422441 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPB40_RS06835 (HPB40_06785) 1416974..1417135 + 162 WP_013095422.1 DUF1127 domain-containing protein -
HPB40_RS06840 (HPB40_06790) 1417222..1418610 - 1389 WP_013095423.1 PLP-dependent aminotransferase family protein -
HPB40_RS06845 (HPB40_06795) 1418713..1419192 + 480 WP_013095424.1 carboxymuconolactone decarboxylase family protein -
HPB40_RS06850 (HPB40_06800) 1419179..1420081 - 903 WP_013095425.1 LysR family transcriptional regulator -
HPB40_RS06855 (HPB40_06805) 1420182..1421597 + 1416 WP_013095426.1 aldehyde dehydrogenase family protein -
HPB40_RS06860 (HPB40_06810) 1421647..1422168 - 522 WP_013095427.1 GNAT family N-acetyltransferase Toxin
HPB40_RS06865 (HPB40_06815) 1422172..1422441 - 270 WP_013095428.1 DUF1778 domain-containing protein Antitoxin
HPB40_RS06870 (HPB40_06820) 1422656..1422979 + 324 WP_023620659.1 antibiotic biosynthesis monooxygenase -
HPB40_RS06875 (HPB40_06825) 1422972..1423370 + 399 WP_013095430.1 amino acid-binding protein -
HPB40_RS06880 (HPB40_06830) 1423342..1424037 + 696 WP_013095431.1 winged helix-turn-helix domain-containing protein -
HPB40_RS06885 (HPB40_06835) 1424241..1424711 + 471 WP_013095432.1 MarR family transcriptional regulator -
HPB40_RS06890 (HPB40_06840) 1424708..1425775 + 1068 WP_095440265.1 HlyD family secretion protein -
HPB40_RS06895 (HPB40_06845) 1425765..1426841 + 1077 WP_013095434.1 DUF2955 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(86-150)

Antitoxin

Vibrio phage ICP1, Orf50

(3-88)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19410.33 Da        Isoelectric Point: 9.2434

>T156840 WP_013095427.1 NZ_CP053568:c1422168-1421647 [Enterobacter cloacae]
VNNMKIGIFSDDVEYDLSHFDCGEESLNTFLTAHLKRQHRGKFLRGYVLVASGEKPRVLGYYTLSGSCFEKAYLPSKTQQ
KRVPYKNVPSVTLGRLAIDKRFQGQGLGELLVTHALKTVYLASFAVGIHGIFVEALNDSAKNFYLKLGFIALQAENENTL
FLPTKTIERLFAD

Download         Length: 522 bp

>T156840 NZ_CP069072:366743-367168 [Mycobacterium tuberculosis]
GTGACTGACCAGCGCTGGCTGATCGACAAGTCGGCGCTGGTGCGGCTCACGGACAGCCCTGACATGGAAATCTGGTCGAA
CCGGATCGAACGCGGCCTGGTACACATCACGGGCGTGACACGCTTGGAAGTAGGGTTCTCGGCCGAATGCGGGGAGATAG
CGCGACGGGAGTTTCGTGAACCGCCGCTGTCTGCGATGCCCGTGGAATACCTAACCCCGAGAATTGAAGACCGTGCGCTC
GAGGTGCAGACCTTGCTTGCCGACCGCGGACACCACCGTGGCCCGTCGATCCCGGATCTGCTCATCGCCGCGACAGCCGA
ACTGTCGGGCTTGACGGTACTGCACGTCGACAAGGACTTTGACGCCATCGCCGCGCTTACCGGTCAGAAAACAGAACGGC
TCACGCATCGCCCGCCTTCCGCTTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10177.54 Da        Isoelectric Point: 5.8652

>AT156840 WP_013095428.1 NZ_CP053568:c1422441-1422172 [Enterobacter cloacae]
MSALKKQRIDLRLTEDDKSLIEEAAAMTNQSISQFMVSTASERAAEVIEQHRRLILNEASWNQVMDAISNPPTPNERLKR
AARRLQDLE

Download         Length: 270 bp

>AT156840 NZ_CP069072:366525-366746 [Mycobacterium tuberculosis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7V7JFS7

Antitoxin

Source ID Structure
AlphaFold DB A0A5E1AP76

References