Detailed information of TA system TA156831

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 18264..18795
• Replicon: plasmid pDCNB
• Accession: NZ_CP053554
• Organism: Diaphorobacter sp. JS3050

Toxin (Protein)

• Gene name: relE
• Uniprot ID: I2ALI9
• Locus tag: HND92_RS18980
• Protein ID: WP_011114071.1
• Coordinates: 18264..18545 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: I2ALI8
• Locus tag: HND92_RS18985
• Protein ID: WP_011114070.1
• Coordinates: 18532..18795 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HND92_RS18960	13519..14484	-	966	WP_011013261.1	P-type conjugative transfer ATPase TrbB	-
HND92_RS18965	14799..15158	-	360	WP_011013260.1	transcriptional regulator	-
HND92_RS18970	15268..15606	+	339	WP_011013259.1	single-stranded DNA-binding protein	-
HND92_RS18975	15655..16878	+	1224	WP_011013257.1	replication initiator protein A	-
HND92_RS18980	18264..18545	-	282	WP_011114071.1	type II toxin-antitoxin system YafQ family toxin	Toxin
HND92_RS18985	18532..18795	-	264	WP_011114070.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
HND92_RS18990	18968..19396	+	429	WP_011114069.1	antirestriction protein	-
HND92_RS18995	19396..19659	+	264	WP_011296181.1	hypothetical protein	-
HND92_RS19000	19671..20879	+	1209	WP_012478186.1	hypothetical protein	-
HND92_RS19005	20896..21153	+	258	WP_012478185.1	hypothetical protein	-
HND92_RS19010	21311..21547	+	237	WP_011255200.1	stable inheritance protein KleA	-
HND92_RS19015	21757..22083	+	327	WP_012478184.1	hypothetical protein	-
HND92_RS19020	22085..22372	+	288	Protein_29	DUF2761 domain-containing protein	-
HND92_RS19025	22616..22924	+	309	WP_011255197.1	transcriptional regulator	-
HND92_RS19030	22921..23685	+	765	WP_013397098.1	ParA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..49405	49405

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-83)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10743.19 Da
• Isoelectric Point: 7.4750

>T156831 WP_011114071.1 NZ_CP053554:c18545-18264 [Diaphorobacter sp. JS3050]
MRQIERTGQFKRDYKREAKGQHRATLDADLVPVLVALADDQPLEPRHRDHALTGDWKDHRDCHVKPDLVLIYQKPDADTL
RLVRLGSHSELGL

Nucleotide

• Length: 282 bp

>T156831 NZ_CP069071:3177141-3177584 [Mycobacterium tuberculosis]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9255.82 Da
• Isoelectric Point: 5.7905

>AT156831 WP_011114070.1 NZ_CP053554:c18795-18532 [Diaphorobacter sp. JS3050]
MAANQLVQTRIDGAIKEEAAAVLAAMGLTVSDAVRLLLTKVAQDKALPFEPLIPNATTIEAMKEARKGKLPRFATVNDLM
ADLHAAD

Nucleotide

• Length: 264 bp

>AT156831 NZ_CP069071:3176897-3177154 [Mycobacterium tuberculosis]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Structures

Toxin

• AlphaFold DB: A0A1U9V4I5

Antitoxin

• AlphaFold DB: A0A1U9V3I2