Detailed information of TA system TA156824

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 324469..324997
• Replicon: plasmid pVEu
• Accession: NZ_CP053542
• Organism: Vibrio europaeus strain NPI-1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A178J4I1
• Locus tag: HOO69_RS16630
• Protein ID: WP_069669844.1
• Coordinates: 324707..324997 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HOO69_RS16625
• Protein ID: WP_171802541.1
• Coordinates: 324469..324717 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HOO69_RS16615	322982..323407	+	426	WP_171802539.1	phage protein	-
HOO69_RS16620	323875..324270	-	396	WP_171802540.1	DUF2391 family protein	-
HOO69_RS16625	324469..324717	+	249	WP_171802541.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HOO69_RS16630	324707..324997	+	291	WP_069669844.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HOO69_RS16635	325195..325617	-	423	WP_171802542.1	hypothetical protein	-
HOO69_RS16640	325749..326285	-	537	WP_069669848.1	hypothetical protein	-
HOO69_RS16645	326448..327077	-	630	WP_171802543.1	hypothetical protein	-
HOO69_RS16650	327105..327215	-	111	WP_084657060.1	DUF3265 domain-containing protein	-
HOO69_RS16655	327219..327635	-	417	WP_171802544.1	hypothetical protein	-
HOO69_RS16660	327684..327836	-	153	WP_171802545.1	DUF3265 domain-containing protein	-
HOO69_RS16665	327802..328101	-	300	WP_171802546.1	hypothetical protein	-
HOO69_RS16670	328159..328332	-	174	WP_171802562.1	DUF3265 domain-containing protein	-
HOO69_RS16675	328329..328607	-	279	WP_069669852.1	hypothetical protein	-
HOO69_RS16680	328980..329135	-	156	WP_004742751.1	hypothetical protein	-
HOO69_RS16685	329135..329545	-	411	WP_171802547.1	hypothetical protein	-
HOO69_RS16690	329542..329970	-	429	WP_171802548.1	M15 family metallopeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..335672	335672

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11260.21 Da
• Isoelectric Point: 10.6296

>T156824 WP_069669844.1 NZ_CP053542:324707-324997 [Vibrio europaeus]
MTYKLDFKKSALKEWKKLGATLQQQFKKKLIERLETPHVQASKLTGADNLYKIKLRQSGYRLVYKVEDDVITVTVLAVGK
RERSDVYRKAMQRLDN

Nucleotide

• Length: 291 bp

>T156824 NZ_CP069071:c2969434-2969111 [Mycobacterium tuberculosis]
TTGACCCACAAGCGCACTAAACGCCAGCCAGCCATCGCCGCAGGGCTCAACGCCCCGCGTCGGAATCGCGTTGGGCGGCA
ACATGGTTGGCCGGCCGACGTTCCGTCCGCCGAGCAGCGCCGCGCCCAACGGCAGCGCGACCTCGAGGCTATCCGCCGAG
CGTACGCCGAGATGGTGGCGACATCACACGAAATCGACGACGACACAGCCGAACTGGCGCTGTTGTCGATGCATCTCGAC
GATGAGCAGCGCCGGCTTGAGGCGGGGATGAAGCTCGGCTGGCATCCGTATCACTTCCCCGACGAACCCGACAGCAAACA
GTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8950.22 Da
• Isoelectric Point: 4.0078

>AT156824 WP_171802541.1 NZ_CP053542:324469-324717 [Vibrio europaeus]
MTTRILADVAASITELKANPMKVATSGCGEPVAVLNRNEPAFYCVPAQAYETLVDRLEDLELLAIAKERELEESISVNID
DL

Nucleotide

• Length: 249 bp

>AT156824 NZ_CP069071:c2969759-2969514 [Mycobacterium tuberculosis]
ATGAGCGGCCACGCGTTGGCTGCTCGGACGTTGCTGGCCGCCGCGGACGAGCTTGTCGGCGGCCCGCCAGTCGAGGCTTC
GGCCGCCGCGCTGGCCGGCGACGCCGCGGGCGCATGGCGGACCGCGGCCGTCGAGCTTGCGCGAGCGTTGGTCCGCGCTG
TGGCGGAGTCGCACGGCGTCGCGGCCGTTTTGTTCGCCGCGACGGCCGCCGCGGCGGCGGCCGTCGACCGGGGTGATCCG
CCGTGA

Structures

Toxin

• AlphaFold DB: A0A178J4I1