Detailed information of TA system TA156818

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): brnT-shpB/BrnT-BrnA
• Location: 2617385..2617851
• Replicon: chromosome
• Accession: NZ_CP053482
• Organism: Thiothrix sp. Ku-5

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: HMY34_RS12945
• Protein ID: WP_202715889.1
• Coordinates: 2617385..2617576 (+)
• Length: 64 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: HMY34_RS12950
• Protein ID: WP_202715890.1
• Coordinates: 2617573..2617851 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HMY34_RS12915	2613123..2613380	+	258	WP_202715885.1	XisI protein	-
HMY34_RS12920	2613299..2615470	-	2172	WP_202715886.1	hypothetical protein	-
HMY34_RS12925	2615467..2616246	-	780	WP_202715887.1	hypothetical protein	-
HMY34_RS12930	2616297..2616482	-	186	WP_202715888.1	hypothetical protein	-
HMY34_RS12935	2616497..2616727	+	231	Protein_2553	XisI protein	-
HMY34_RS12940	2616956..2617303	+	348	WP_202719199.1	XisI protein	-
HMY34_RS12945	2617385..2617576	+	192	WP_202715889.1	BrnT family toxin	Toxin
HMY34_RS12950	2617573..2617851	+	279	WP_202715890.1	BrnA antitoxin family protein	Antitoxin
HMY34_RS12955	2617862..2618008	+	147	Protein_2557	XisI protein	-
HMY34_RS12960	2618013..2618213	-	201	WP_202715891.1	hypothetical protein	-
HMY34_RS12965	2618474..2619058	+	585	WP_202715892.1	SLATT domain-containing protein	-
HMY34_RS12970	2619055..2619243	+	189	WP_202715893.1	hypothetical protein	-
HMY34_RS12975	2619250..2619666	-	417	WP_202715894.1	hypothetical protein	-
HMY34_RS12980	2619663..2620157	-	495	WP_202715895.1	transposase family protein	-
HMY34_RS12985	2620271..2621488	+	1218	WP_202719200.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-56)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (28-84)

Sequences

Toxin

Protein

• Length: 64 a.a.
• Molecular weight: 7543.24 Da
• Isoelectric Point: 4.2813

>T156818 WP_202715889.1 NZ_CP053482:2617385-2617576 [Thiothrix sp. Ku-5]
MYRKFEWDENKNRTNQQKHGVDFDTASYVFDDPNFLSEQDRIVDGEERWQTIGLVGGFEEKIL

Nucleotide

• Length: 192 bp

>T156818 NZ_CP069071:2802201-2802626 [Mycobacterium tuberculosis]
GTGGCGCTGCTCGACGTCAACGCATTGGTCGCGCTGGCGTGGGACTCACACATCCACCACGCCCGGATCCGCGAGTGGTT
TACCGCCAACGCCACGCTCGGCTGGGCGACTTGCCCGCTCACCGAAGCCGGCTTCGTGCGGGTGTCGACGAACCCAAAAG
TACTTCCCAGCGCGATCGGGATCGCAGACGCTCGACGGGTCCTCGTGGCACTACGCGCCGTGGGAGGCCACCGCTTCCTG
GCTGACGACGTATCGCTCGTCGATGACGATGTTCCGTTGATCGTCGGTTATCGCCAGGTGACCGACGCCCATCTGCTGAC
ACTCGCCCGCCGGCGCGGCGTCCGCCTGGTCACCTTCGACGCCGGTGTCTTCACCCTCGCCCAACAACGCCCCAAGACGC
CAGTGGAGCTGCTGACCATCCTCTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10313.76 Da
• Isoelectric Point: 6.3447

>AT156818 WP_202715890.1 NZ_CP053482:2617573-2617851 [Thiothrix sp. Ku-5]
MNRQLSDKQIAMLKALADKPDEDIDLSDIPEAGDDAWKNAVPGEFFRPIKKQITVRIDADVLAWLKSSGNGYQTRLNQVL
REAMEKTGKRAA

Nucleotide

• Length: 279 bp

>AT156818 NZ_CP069071:2801974-2802195 [Mycobacterium tuberculosis]
GTGCGAACGACACTTGACCTTGACGACGATGTGATCGCCGCGGCACGTGAACTTGCCTCCAGCCAGCGCCGCTCGCTCGG
CTCGGTGATTTCCGAACTCGCACGCCGTGGTCTCATGCCCGGACGCGTCGAGGCTGACGACGGGCTGCCGGTGATCCGCG
TTCCAGCCGGGACCCCGCCGATCACACCGGAGATGGTCCGTCGCGCGCTCGATGAGGACTGA