Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-Phd
Location 56157..56848 Replicon plasmid pRltCC275eF
Accession NZ_CP053440
Organism Rhizobium leguminosarum bv. trifolii strain CC275e

Toxin (Protein)


Gene name vapC Uniprot ID A0A6G8VST5
Locus tag RLCC275e_RS23880 Protein ID WP_003562524.1
Coordinates 56157..56585 (-) Length 143 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A6G8VSU1
Locus tag RLCC275e_RS23885 Protein ID WP_003562523.1
Coordinates 56582..56848 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RLCC275e_RS23860 53050..53889 + 840 WP_003562529.1 transporter substrate-binding domain-containing protein -
RLCC275e_RS23865 54007..54669 + 663 WP_033183217.1 amino acid ABC transporter permease -
RLCC275e_RS23870 54674..55333 + 660 WP_011648868.1 amino acid ABC transporter permease -
RLCC275e_RS23875 55346..56116 + 771 WP_003562525.1 amino acid ABC transporter ATP-binding protein -
RLCC275e_RS23880 56157..56585 - 429 WP_003562524.1 type II toxin-antitoxin system VapC family toxin Toxin
RLCC275e_RS23885 56582..56848 - 267 WP_003562523.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
RLCC275e_RS23890 57108..57851 + 744 WP_033183216.1 GntR family transcriptional regulator -
RLCC275e_RS23895 57855..58640 + 786 WP_003562521.1 transporter substrate-binding domain-containing protein -
RLCC275e_RS23900 58722..59393 + 672 WP_033183215.1 amino acid ABC transporter permease -
RLCC275e_RS23905 59390..60049 + 660 WP_003562519.1 amino acid ABC transporter permease -
RLCC275e_RS23910 60027..60752 + 726 WP_033183214.1 amino acid ABC transporter ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..971705 971705


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(4-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(9-66)


Sequences

Toxin        


Download         Length: 143 a.a.        Molecular weight: 15812.09 Da        Isoelectric Point: 4.7948

>T156802 WP_003562524.1 NZ_CP053440:c56585-56157 [Rhizobium leguminosarum bv. trifolii]
VRLLLDTNVLSEVTRPRPDAHVLQWLDSLDEDRSFISVVSIAEIRRGVALMESGRKRDALAEWLAQDLPQRFEQRVIPVD
QPVAIAWGDLMGLAKRSGRGLSSMDGLIAATAIAHDLTLATRNIKDFEGFGIELVDPWAERP

Download         Length: 429 bp

>T156802 NZ_CP069071:1540887-1541708 [Mycobacterium tuberculosis]
GTGGTGGTAGCCCTCGTAGGCTCAGCAATCGTGGACTTGCATTCACGACCACCGTGGTCGAACAACGCGGTGCGTCGTCT
TGGCGTGGCACTGCGCGACGGAGTTGACCCGCCGGTCGACTGCCCGTCGTACGCCGAGGTGATGCTGTGGCATGCGGACT
TGGCCGCCGAAGTCCAGGACCGGATCGAGGGCCGGAGTTGGTCTGCGTCGGAGTTATTGGTTACCTCACGTGCGAAGAGC
CAAGACACCCTGCTAGCAAAGCTGCGGCGTCGGCCTTACCTGCAACTGAACACCATCCAAGACATCGCAGGTGTCCGCAT
CGATGCCGACCTCCTGCTGGGCGAGCAGACGAGACTTGCTCGCGAGATCGCCGACCACTTCGGTGCTGACCAGCCCGCTA
TTCATGATCTGCGTGACCACCCGCACGCCGGCTACCGGGCCGTTCATGTCTGGCTTCGGTTACCTGCCGGTCGTGTCGAG
ATACAGATTCGCACCATTTTGCAGAGCCTGTGGGCCAACTTCTACGAGCTTCTCGCTGACGCGTACGGTCGGGGCATCCG
CTATGACGAGCGGCCGGAGCAGCTAGCGGCCGGCGTTGTCCCGGCACAGCTTCAAGAGCTGGTAGGGGTTATGCAAGACG
CTTCAGCGGATCTGGCGATGCATGAAGCCGAGTGGCAACACTGTGCAGAGATCGAATACCCCGGCCAGCGGGCGATGGCG
CTTGGCGAGGCGAGCAAGAACAAGGCGACGGTGCTCGCAACGACCAAGTTTAGGCTGGAAAGGGCCATCAATGAGGCCGA
GTCGGCAGGGGGAGGTGGGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9994.36 Da        Isoelectric Point: 10.0842

>AT156802 WP_003562523.1 NZ_CP053440:c56848-56582 [Rhizobium leguminosarum bv. trifolii]
MQPSRPEDKSWTVASAKAKLSEVIERAQATPQTIMRNGKPSVVVVSAEEWQRKTARKGTLAEFLMQSPLRGADLDLERQR
DEPRDFQL

Download         Length: 267 bp

>AT156802 NZ_CP069071:1541701-1541937 [Mycobacterium tuberculosis]
GTGGGTGAGGTGGCTGGCTATGTCGTCGAATACAACCGGCGCACCCACGTGCGTCGCATCACCGAGTTCGCCACCCCGCA
AGAAGCGATGGAGCACCGGTTGAAGCTGGAAGCCGAGCGCACCGACAGCAATATCGAGATCGTTGCGCTCGTCAGTAAGT
CGTTGGGAACCCTGAAGCAAACGCATTCGCGGTACTTCACTGGTGAAGAGCTGAACGTCGGAAACGGCGCGCGGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6G8VST5

Antitoxin

Source ID Structure
AlphaFold DB A0A6G8VSU1

References