Detailed information of TA system TA156752

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 5544334..5545554
• Replicon: chromosome
• Accession: NZ_CP053390
• Organism: Pseudomonas aeruginosa strain TL1285

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0H2Z8V4
• Locus tag: HMJ40_RS26110
• Protein ID: WP_003110488.1
• Coordinates: 5544334..5544912 (-)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HMJ40_RS26115
• Protein ID: WP_021264660.1
• Coordinates: 5545000..5545554 (-)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HMJ40_RS26095 (HMJ40_26105)	5540163..5542334	+	2172	WP_003088105.1	type I secretion system permease/ATPase	-
HMJ40_RS26100 (HMJ40_26110)	5542324..5543511	+	1188	WP_216787121.1	HlyD family efflux transporter periplasmic adaptor subunit	-
HMJ40_RS26105 (HMJ40_26115)	5543709..5544337	+	629	Protein_5155	LysE family translocator	-
HMJ40_RS26110 (HMJ40_26120)	5544334..5544912	-	579	WP_003110488.1	HD domain-containing protein	Toxin
HMJ40_RS26115 (HMJ40_26125)	5545000..5545554	-	555	WP_021264660.1	helix-turn-helix transcriptional regulator	Antitoxin
HMJ40_RS26120 (HMJ40_26130)	5545712..5547907	-	2196	WP_023091929.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
HMJ40_RS26125 (HMJ40_26135)	5547912..5548373	-	462	WP_003102736.1	(2Fe-2S)-binding protein	-
HMJ40_RS26130 (HMJ40_26140)	5548704..5549027	+	324	WP_003102734.1	multidrug efflux SMR transporter	-
HMJ40_RS26135 (HMJ40_26145)	5549040..5549438	+	399	WP_003088123.1	NADH-quinone oxidoreductase subunit A	-
HMJ40_RS26140 (HMJ40_26150)	5549435..5549986	-	552	WP_003132721.1	XRE family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• 5'-deoxynucleotidase YfbR/HDDC2 (7-188)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21913.75 Da
• Isoelectric Point: 5.3655

>T156752 WP_003110488.1 NZ_CP053390:c5544912-5544334 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDTPLRDRLLALWDEYERGETAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T156752 NZ_CP069070:2272145-2272462 [Mycobacterium tuberculosis]
ATGACGCGCAGGCTGCGCGTCCATAACGGGGTTGAAGACGACCTATTCGAGGCGTTTTCCTACTACGCGGACGCGGCGCC
AGATCAGATCGATCGGCTTTACAACTTGTTTGTCGATGCCGTGACGAAGCGGATTCCGCAGGCTCCGAACGCGTTTGCGC
CGTTATTCAAGCACTATCGACACATCTACCTCCGGCCCTTCAGGTACTACGTTGCCTATCGGACGACCGACGAGGCTATC
GACATACTGGCTGTTCGGCACGGAATGGAGAACCCGAACGCGGTCGAGGCTGAGATCTCTGGCCGCACCTTCGAGTGA

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20484.66 Da
• Isoelectric Point: 8.1590

>AT156752 WP_021264660.1 NZ_CP053390:c5545554-5545000 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRIKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLESAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT156752 NZ_CP069070:2271933-2272148 [Mycobacterium tuberculosis]
GTGGTGGTCAACCGGGCATTGCTGGCGAGCGTCGACGCACTGTCGCGTGATGAGCAGATTGAGCTCGTCGAGCACATCAA
CGGAAACCTAGCCGAGGGCATGCATATCAGCGAGGCCAACCAGGCGCTCATCGAAGCGCGGGCCAATGACACCGACGATG
CTCATTGGTCCACCATTGATGACTTCGACAAGCGGATCCGCGCCCGGCTCGGATGA

Structures

Toxin

• AlphaFold DB: A0A0H2Z8V4