Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /Xre(antitoxin)
Location 4556762..4558020 Replicon chromosome
Accession NZ_CP053390
Organism Pseudomonas aeruginosa strain TL1285

Toxin (Protein)


Gene name - Uniprot ID A0A509JCW5
Locus tag HMJ40_RS21365 Protein ID WP_021264413.1
Coordinates 4557265..4558020 (+) Length 252 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID V6AJU7
Locus tag HMJ40_RS21360 Protein ID WP_003086329.1
Coordinates 4556762..4557130 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HMJ40_RS21340 (HMJ40_21350) 4552926..4553201 + 276 WP_023088486.1 zinc ribbon domain-containing protein -
HMJ40_RS21345 (HMJ40_21355) 4553204..4553680 - 477 WP_019486191.1 elongation factor GreAB -
HMJ40_RS21350 (HMJ40_21360) 4553759..4555348 + 1590 WP_003123191.1 aldehyde dehydrogenase family protein -
HMJ40_RS21355 (HMJ40_21365) 4555404..4556689 + 1286 Protein_4210 FAD-binding oxidoreductase -
HMJ40_RS21360 (HMJ40_21370) 4556762..4557130 + 369 WP_003086329.1 DUF2384 domain-containing protein Antitoxin
HMJ40_RS21365 (HMJ40_21375) 4557265..4558020 + 756 WP_021264413.1 RES family NAD+ phosphorylase Toxin
HMJ40_RS21370 (HMJ40_21380) 4558790..4560151 + 1362 WP_073647918.1 DNA recombination protein RmuC -
HMJ40_RS21375 (HMJ40_21385) 4560306..4562849 - 2544 WP_216787050.1 acyl-homoserine-lactone acylase QuiP -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(63-207)

Antitoxin

Cro/C1-type helix-turn-helix domain

(23-47)


Sequences

Toxin        


Download         Length: 252 a.a.        Molecular weight: 28217.33 Da        Isoelectric Point: 8.0554

>T156751 WP_021264413.1 NZ_CP053390:4557265-4558020 [Pseudomonas aeruginosa]
VSEIWRQCKGERHVRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLQTAMAESAYYRCVLWSGMAVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAIRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Download         Length: 756 bp

>T156751 NZ_CP069070:c1861660-1861235 [Mycobacterium tuberculosis]
GTGGCGCTGCTCGACGTCAACGCATTGGTCGCGCTGGCGTGGGACTCACACATCCACCACGCCCGGATCCGCGAGTGGTT
TACCGCCAACGCCACGCTCGGCTGGGCGACTTGCCCGCTCACCGAAGCCGGCTTCGTGCGGGTGTCGACGAACCCAAAAG
TACTTCCCAGCGCGATCGGGATCGCAGACGCTCGACGGGTCCTCGTGGCACTACGCGCCGTGGGAGGCCACCGCTTCCTG
GCTGACGACGTATCGCTCGTCGATGACGATGTTCCGTTGATCGTCGGTTATCGCCAGGTGACCGACGCCCATCTGCTGAC
ACTCGCCCGCCGGCGCGGCGTCCGCCTGGTCACCTTCGACGCCGGTGTCTTCACCCTCGCCCAACAACGCCCCAAGACGC
CAGTGGAGCTGCTGACCATCCTCTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13367.63 Da        Isoelectric Point: 9.9874

>AT156751 WP_003086329.1 NZ_CP053390:4556762-4557130 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Download         Length: 369 bp

>AT156751 NZ_CP069070:c1861887-1861666 [Mycobacterium tuberculosis]
GTGCGAACGACACTTGACCTTGACGACGATGTGATCGCCGCGGCACGTGAACTTGCCTCCAGCCAGCGCCGCTCGCTCGG
CTCGGTGATTTCCGAACTCGCACGCCGTGGTCTCATGCCCGGACGCGTCGAGGCTGACGACGGGCTGCCGGTGATCCGCG
TTCCAGCCGGGACCCCGCCGATCACACCGGAGATGGTCCGTCGCGCGCTCGATGAGGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A509JCW5

Antitoxin

Source ID Structure
AlphaFold DB V6AJU7

References