Detailed information of TA system TA156750

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE(toxin)
• Location: 4167478..4168086
• Replicon: chromosome
• Accession: NZ_CP053390
• Organism: Pseudomonas aeruginosa strain TL1285

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: A0A509JBR4
• Locus tag: HMJ40_RS19490
• Protein ID: WP_031638390.1
• Coordinates: 4167739..4168086 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: A0A0B0C355
• Locus tag: HMJ40_RS19485
• Protein ID: WP_003114155.1
• Coordinates: 4167478..4167729 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HMJ40_RS19465 (HMJ40_19480)	4163116..4163466	+	351	WP_003133726.1	DUF2523 family protein	-
HMJ40_RS19470 (HMJ40_19485)	4163468..4164730	+	1263	WP_033953311.1	zonular occludens toxin domain-containing protein	-
HMJ40_RS19475 (HMJ40_19490)	4164989..4166281	+	1293	WP_003123045.1	hypothetical protein	-
HMJ40_RS19480 (HMJ40_19495)	4166281..4167264	+	984	WP_034014422.1	tyrosine-type recombinase/integrase	-
HMJ40_RS19485 (HMJ40_19500)	4167478..4167729	+	252	WP_003114155.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
HMJ40_RS19490 (HMJ40_19505)	4167739..4168086	+	348	WP_031638390.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HMJ40_RS19500 (HMJ40_19515)	4168413..4169315	-	903	WP_216787019.1	(R)-3-hydroxydecanoyl-ACP:CoA transacylase	-
HMJ40_RS19505 (HMJ40_19520)	4169767..4170504	-	738	WP_021264273.1	murein L,D-transpeptidase catalytic domain family protein	-
HMJ40_RS19510 (HMJ40_19525)	4170584..4171625	-	1042	Protein_3847	L,D-transpeptidase	-
HMJ40_RS19515 (HMJ40_19530)	4171761..4172453	-	693	WP_015503463.1	16S rRNA pseudouridine(516) synthase	-
HMJ40_RS19520 (HMJ40_19535)	4172453..4172725	-	273	WP_021264275.1	cysteine-rich CWC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	catB7	-	4146035..4172665	26630

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-100)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13018.79 Da
• Isoelectric Point: 4.4212

>T156750 WP_031638390.1 NZ_CP053390:4167739-4168086 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLVPFQGEQAAFQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEAAVILVLRQKQSVEQQLIRYCLVGPIE

Nucleotide

• Length: 348 bp

>T156750 NZ_CP069070:1815284-1815703 [Mycobacterium tuberculosis]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9442.79 Da
• Isoelectric Point: 9.4808

>AT156750 WP_003114155.1 NZ_CP053390:4167478-4167729 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Nucleotide

• Length: 252 bp

>AT156750 NZ_CP069070:1815063-1815287 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Structures

Toxin

• AlphaFold DB: A0A509JBR4

Antitoxin

• AlphaFold DB: A0A0B0C355