Detailed information of TA system TA156747

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 3029656..3030161
• Replicon: chromosome
• Accession: NZ_CP053390
• Organism: Pseudomonas aeruginosa strain TL1285

Toxin (Protein)

• Gene name: parE
• Uniprot ID: V6A7K8
• Locus tag: HMJ40_RS14090
• Protein ID: WP_003083773.1
• Coordinates: 3029656..3029937 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A1C7BDS9
• Locus tag: HMJ40_RS14095
• Protein ID: WP_003083775.1
• Coordinates: 3029934..3030161 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HMJ40_RS14065 (HMJ40_14080)	3024907..3026256	+	1350	WP_216787392.1	C4-dicarboxylate transporter DctA	-
HMJ40_RS14070 (HMJ40_14085)	3026305..3026991	+	687	WP_003083762.1	FadR/GntR family transcriptional regulator	-
HMJ40_RS14075 (HMJ40_14090)	3027092..3027826	+	735	WP_003110658.1	GntR family transcriptional regulator	-
HMJ40_RS14080 (HMJ40_14095)	3028006..3028416	+	411	WP_003110659.1	aegerolysin family protein	-
HMJ40_RS14085 (HMJ40_14100)	3028448..3029356	-	909	WP_003083769.1	LysR family transcriptional regulator	-
HMJ40_RS14090 (HMJ40_14105)	3029656..3029937	-	282	WP_003083773.1	type II toxin-antitoxin system toxin ParE	Toxin
HMJ40_RS14095 (HMJ40_14110)	3029934..3030161	-	228	WP_003083775.1	CopG family ribbon-helix-helix protein	Antitoxin
HMJ40_RS14100 (HMJ40_14115)	3030337..3030957	-	621	WP_003101226.1	hypothetical protein	-
HMJ40_RS14105 (HMJ40_14120)	3031058..3031558	+	501	WP_003162763.1	LEA type 2 family protein	-
HMJ40_RS14110 (HMJ40_14125)	3031631..3031972	+	342	WP_003101229.1	zinc ribbon domain-containing protein YjdM	-
HMJ40_RS14115 (HMJ40_14130)	3032055..3033482	-	1428	WP_003083784.1	GABA permease	-
HMJ40_RS14120 (HMJ40_14135)	3033651..3035144	-	1494	WP_003101230.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-87)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10462.19 Da
• Isoelectric Point: 10.0435

>T156747 WP_003083773.1 NZ_CP053390:c3029937-3029656 [Pseudomonas aeruginosa]
MSLKWTRKAAADLDAIYDHYVVLIGPEKALKAVQDIVEQVKPLQQVANQGAGRPSEVPGVRTLTLERWPFSAPFRVKGKE
IQILRIDRVEITP

Nucleotide

• Length: 282 bp

>T156747 NZ_CP069070:1799192-1799587 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8556.58 Da
• Isoelectric Point: 5.5740

>AT156747 WP_003083775.1 NZ_CP053390:c3030161-3029934 [Pseudomonas aeruginosa]
MSTVVSFRADDALVAALDELARATHRDRPYHLRQALAQYLERQQWQVAAIDEGLADANAGRLLEHTEIEKRWGLQ

Nucleotide

• Length: 228 bp

>AT156747 NZ_CP069070:1798950-1799195 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Structures

Toxin

• PDB: 6XRW

Antitoxin

• AlphaFold DB: A0A1C7BDS9