Detailed information of TA system TA156742

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 3766168..3767915
• Replicon: chromosome
• Accession: NZ_CP053382
• Organism: Halomonas sp. MCCC 1A13316

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: HNO52_RS17405
• Protein ID: WP_197566487.1
• Coordinates: 3766168..3766824 (+)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: HNO52_RS17410
• Protein ID: WP_197566488.1
• Coordinates: 3766821..3767915 (+)
• Length: 365 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HNO52_RS17395	3762967..3764304	+	1338	WP_197566485.1	restriction endonuclease subunit S	-
HNO52_RS17400	3764353..3766164	+	1812	WP_197566486.1	DUF262 domain-containing protein	-
HNO52_RS17405	3766168..3766824	+	657	WP_197566487.1	DUF4433 domain-containing protein	Toxin
HNO52_RS17410	3766821..3767915	+	1095	WP_197566488.1	macro domain-containing protein	Antitoxin
HNO52_RS17415	3767980..3769191	+	1212	WP_197566489.1	hypothetical protein	-
HNO52_RS17420	3769307..3772678	+	3372	WP_197566490.1	type I restriction endonuclease subunit R	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-217)

Antitoxin

• Macro domain (18-129)

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 24682.39 Da
• Isoelectric Point: 8.0931

>T156742 WP_197566487.1 NZ_CP053382:3766168-3766824 [Halomonas sp. MCCC 1A13316]
MATDLSRSLNPRKALIFRITHRDNLPWLLANGLYCPSAAEQAPRFITIGKQDLIDKRATRVVPVPPGGVLSDYIPFYFTP
ASMMLFNIVTGHGVQQQRREDIVVMVSSLPRLEQLGLPFLFSDRHAYLANAEFSDRLEDLADFVPWGLLQAKDFKRDLEN
PDKTDRYQAEALIHHHLPASALLGIGTYTDEVRVAVRAQVAAQQLSLKVVTRPNWFFQ

Nucleotide

• Length: 657 bp

>T156742 NZ_CP069070:c1390365-1390072 [Mycobacterium tuberculosis]
GTGAGCGACGACCATCCCTACCACGTGGCGATCACCGCGACAGCGGCACGCGACCTGCAACGCTTACCCGAAAAGATCGC
CGCCGCATGTGTCGAGTTTGTTTTCGGACCGCTGCTTAACAACCCGCATAGGTTGGGCAAGCCGCTGCGCAATGACCTTG
AAGGCCTCCACTCAGCCCGCCGCGGTGATTACCGCGTCGTCTACGCCATCGACGACGGCCACCACCGAGTCGAGATCATC
CACATCGCTCGTCGCAGTGCCAGCTACCGAATGAACCCGTGCCGGCCACGTTAA

Antitoxin

Protein

• Length: 365 a.a.
• Molecular weight: 41357.65 Da
• Isoelectric Point: 6.3531

>AT156742 WP_197566488.1 NZ_CP053382:3766821-3767915 [Halomonas sp. MCCC 1A13316]
MIEYTRGNLLEADVEALVNTVNTVGVMGKGIALMFKEAFPENFAAYEAACKHQQVVTGRMFITERDALLGPRWIINFPTK
QHWRAKTRVEWIESGLEDLKRVIREKGIRSIALPPLGCGNGGLDWAMVRPRIEAALAELDNVRVLVFGPTVEYQNVAKRS
GVEKLTPARAMIAELVRRYWVLGIECSLLEVQKLAWLMEQRIIAHGLENPLKLEFQPHRYGPYSDRLRHLLNGLDGSYLH
SEKRINDATPEEVVWFDESRRDRLHVYLRSAEAHPYAGVLEEVDALIDGFQSPLGMEVLATLDWLISQEHVEPTVEAIKE
GLRHWPDDIAGQRKLRLFSDRLIALALERLREGSPTTQVISTAL

Nucleotide

• Length: 1095 bp

>AT156742 NZ_CP069070:c1390631-1390362 [Mycobacterium tuberculosis]
ATGGCTGTTGTCCCACTGGGCGAAGTCCGCAATCGCCTCTCTGAGTACGTCGCCGAAGTTGAGCTGACACACGAGCGCAT
CACGATAACCCGGCACGGTCATCCGGCGGCGGTATTGATCTCGGCCGATGACCTGGCGTCCATCGAGGAAACGCTGGAGG
TGCTACGCACCCCTGGCGCCAGCGAGGCCATTCGTGAAGGCCTCGCCGATGTTGCCGCAGGGCGCTTCGTGAGCAACGAC
GAGATCCGCAACCGTTACACCGCGCGGTGA