Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 1271577..1272494 Replicon chromosome
Accession NZ_CP053377
Organism Bacillus velezensis strain EN01

Toxin (Protein)


Gene name spoIISA Uniprot ID A0A6A8LGT8
Locus tag HNO13_RS06465 Protein ID WP_003154806.1
Coordinates 1271748..1272494 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID I2HQ14
Locus tag HNO13_RS06460 Protein ID WP_003154807.1
Coordinates 1271577..1271747 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HNO13_RS06425 1266809..1268431 + 1623 WP_003154821.1 pyocin knob domain-containing protein -
HNO13_RS06430 1268444..1268815 + 372 WP_014304856.1 hypothetical protein -
HNO13_RS06435 1268821..1269018 + 198 WP_003154819.1 XkdX family protein -
HNO13_RS06440 1269075..1269836 + 762 WP_046559521.1 hypothetical protein -
HNO13_RS06445 1269888..1270151 + 264 WP_003154815.1 hemolysin XhlA family protein -
HNO13_RS06450 1270165..1270428 + 264 WP_003154813.1 phage holin -
HNO13_RS06455 1270442..1271320 + 879 WP_024085195.1 N-acetylmuramoyl-L-alanine amidase -
HNO13_RS06460 1271577..1271747 - 171 WP_003154807.1 type II toxin-antitoxin system SpoIISB family antitoxin Antitoxin
HNO13_RS06465 1271748..1272494 - 747 WP_003154806.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
HNO13_RS06470 1272599..1273597 - 999 WP_003154805.1 inorganic phosphate transporter -
HNO13_RS06475 1273610..1274227 - 618 WP_003154804.1 DUF47 domain-containing protein -
HNO13_RS06480 1274513..1275829 - 1317 WP_003154801.1 amino acid permease -
HNO13_RS06485 1276153..1277103 + 951 WP_024085196.1 ring-cleaving dioxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1224602..1329821 105219


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-239)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29034.50 Da        Isoelectric Point: 4.6947

>T156721 WP_003154806.1 NZ_CP053377:c1272494-1271748 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQDRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Download         Length: 747 bp

>T156721 NZ_CP069070:366075-366377 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6676.74 Da        Isoelectric Point: 10.9273

>AT156721 WP_003154807.1 NZ_CP053377:c1271747-1271577 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Download         Length: 171 bp

>AT156721 NZ_CP069070:366425-366646 [Mycobacterium tuberculosis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6A8LGT8

Antitoxin

Source ID Structure
AlphaFold DB I2HQ14

References