156720

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 508622..509259 Replicon chromosome
Accession NZ_CP053377
Organism Bacillus velezensis strain EN01

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag HNO13_RS02435 Protein ID WP_003156187.1
Coordinates 508909..509259 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag HNO13_RS02430 Protein ID WP_003156188.1
Coordinates 508622..508903 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HNO13_RS02410 504987..505586 - 600 WP_003156193.1 rhomboid family intramembrane serine protease -
HNO13_RS02415 505679..506044 + 366 WP_014304402.1 holo-ACP synthase -
HNO13_RS02420 506209..507216 + 1008 WP_003156191.1 outer membrane lipoprotein carrier protein LolA -
HNO13_RS02425 507333..508502 + 1170 WP_003156189.1 alanine racemase -
HNO13_RS02430 508622..508903 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
HNO13_RS02435 508909..509259 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
HNO13_RS02440 509377..510198 + 822 WP_014304404.1 STAS domain-containing protein -
HNO13_RS02445 510203..510568 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
HNO13_RS02450 510571..510972 + 402 WP_003156178.1 anti-sigma regulatory factor -
HNO13_RS02455 510984..511991 + 1008 WP_003156177.1 PP2C family protein-serine/threonine phosphatase -
HNO13_RS02460 512055..512384 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
HNO13_RS02465 512381..512863 + 483 WP_003156173.1 anti-sigma B factor RsbW -
HNO13_RS02470 512829..513617 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
HNO13_RS02475 513617..514219 + 603 WP_003156170.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T156720 WP_003156187.1 NZ_CP053377:508909-509259 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T156720 NZ_CP069070:366075-366377 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT156720 WP_003156188.1 NZ_CP053377:508622-508903 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT156720 NZ_CP069070:365851-366078 [Mycobacterium tuberculosis]
ATGACGAAAGAGAAGATCTCCGTGACGGTGGACGCGGCCGTCCTCGCGGCGATCGACGCGGACGCCAGGGCGGCGGGTTT
GAATCGGTCGGAAATGATTGAGCAGGCACTGCGCAACGAGCACCTGCGTGTCGCTCTGCGCGATTACACGGCTAAAACCG
TACCGGCGTTGGACATCGATGCCTACGCACAGCGGGTGTACCAGGCGAACCGGGCGGCCGGAAGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References