Detailed information of TA system TA156719

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1271324..1272241
• Replicon: chromosome
• Accession: NZ_CP053376
• Organism: Bacillus amyloliquefaciens strain WF02

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: A0A6A8LGT8
• Locus tag: HNO12_RS06470
• Protein ID: WP_003154806.1
• Coordinates: 1271495..1272241 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: HNO12_RS06465
• Protein ID: WP_003154807.1
• Coordinates: 1271324..1271494 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HNO12_RS06430	1266556..1268178	+	1623	WP_003154821.1	pyocin knob domain-containing protein	-
HNO12_RS06435	1268191..1268562	+	372	WP_014304856.1	hypothetical protein	-
HNO12_RS06440	1268568..1268765	+	198	WP_003154819.1	XkdX family protein	-
HNO12_RS06445	1268822..1269583	+	762	WP_046559521.1	hypothetical protein	-
HNO12_RS06450	1269635..1269898	+	264	WP_003154815.1	hemolysin XhlA family protein	-
HNO12_RS06455	1269912..1270175	+	264	WP_003154813.1	phage holin	-
HNO12_RS06460	1270189..1271067	+	879	WP_024085195.1	N-acetylmuramoyl-L-alanine amidase	-
HNO12_RS06465	1271324..1271494	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
HNO12_RS06470	1271495..1272241	-	747	WP_003154806.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
HNO12_RS06475	1272346..1273344	-	999	WP_003154805.1	inorganic phosphate transporter	-
HNO12_RS06480	1273357..1273974	-	618	WP_003154804.1	DUF47 domain-containing protein	-
HNO12_RS06485	1274260..1275576	-	1317	WP_003154801.1	amino acid permease	-
HNO12_RS06490	1275900..1276850	+	951	WP_024085196.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1227533..1329568	102035

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29034.50 Da
• Isoelectric Point: 4.6947

>T156719 WP_003154806.1 NZ_CP053376:c1272241-1271495 [Bacillus amyloliquefaciens]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQDRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T156719 NZ_CP069070:71507-71908 [Mycobacterium tuberculosis]
GTGGATGAATGTGTAGTCGACGCGGCGGCCGTGGTTGACGCTCTCGCCGGCAAGGGCGCCAGCGCGATCGTTCTGCGCGG
TTTGCTCAAGGAGTCGATTTCTAACGCGCCGCATTTGCTGGACGCAGAGGTCGGACATGCACTCCGCCGCGCCGTGCTCA
GCGACGAAATCTCCGAAGAGCAGGCTCGCGCCGCGTTGGATGCCTTGCCTTATCTCATCGACAATCGTTACCCGCACAGC
CCACGACTGATCGAATACACATGGCAGCTAAGGCACAACGTCACGTTCTACGACGCCCTTTACGTCGCACTGGCCACCGC
ACTGGATGTCCCGCTGCTCACGGGCGACTCGCGGCTTGCGGCCGCGCCGGGCCTTCCGTGCGAAATCAAACTCGTTCGGT
GA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT156719 WP_003154807.1 NZ_CP053376:c1271494-1271324 [Bacillus amyloliquefaciens]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT156719 NZ_CP069070:71275-71514 [Mycobacterium tuberculosis]
ATGGCTACCATTCAAGTTCGGGATTTGCCCGAAGATGTCGCCGAAACCTATCGACGGCGCGCCACCGCAGCGGGGCAGTC
GCTGCAGACGTATATGCGCACCAAGCTCATCGAAGGGGTGCGGGGCCGAGACAAGGCCGAGGCAATCGAGATCCTGGAAC
AGGCGCTCGCCAGCACTGCCAGCCCAGGCATCAGCCGGGAGACCATCGAGGCATCCCGGCGGGAGCTCAGGGGTGGATGA

Structures

Toxin

• AlphaFold DB: A0A6A8LGT8

Antitoxin

• AlphaFold DB: I2HQ14