Detailed information of TA system TA156718

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 509033..509670
• Replicon: chromosome
• Accession: NZ_CP053376
• Organism: Bacillus amyloliquefaciens strain WF02

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: HNO12_RS02435
• Protein ID: WP_003156187.1
• Coordinates: 509320..509670 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: HNO12_RS02430
• Protein ID: WP_003156188.1
• Coordinates: 509033..509314 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HNO12_RS02410	505398..505997	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
HNO12_RS02415	506090..506455	+	366	WP_014304402.1	holo-ACP synthase	-
HNO12_RS02420	506620..507627	+	1008	WP_003156191.1	outer membrane lipoprotein carrier protein LolA	-
HNO12_RS02425	507744..508913	+	1170	WP_003156189.1	alanine racemase	-
HNO12_RS02430	509033..509314	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
HNO12_RS02435	509320..509670	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
HNO12_RS02440	509788..510609	+	822	WP_139850290.1	STAS domain-containing protein	-
HNO12_RS02445	510614..510979	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
HNO12_RS02450	510982..511383	+	402	WP_003156178.1	anti-sigma regulatory factor	-
HNO12_RS02455	511395..512402	+	1008	WP_003156177.1	PP2C family protein-serine/threonine phosphatase	-
HNO12_RS02460	512466..512795	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
HNO12_RS02465	512792..513274	+	483	WP_003156173.1	anti-sigma B factor RsbW	-
HNO12_RS02470	513240..514028	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
HNO12_RS02475	514028..514630	+	603	WP_003156170.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T156718 WP_003156187.1 NZ_CP053376:509320-509670 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T156718 NZ_CP069069:3842819-3843229 [Mycobacterium tuberculosis]
GTGATCTATATGGACACCTCGGCCCTGACTAAGCTGCTCATCTCCGAGCCCGAGACGACCGAACTGCGGACATGGCTGAC
CGCGCAAAGCGGCCAGGGCGAGGACGCGGCGACAAGCACCCTTGGCCGGGTCGAGTTGATGAGAGTCGTTGCCCGATACG
GACAACCAGGCCAAACTGAGCGTGCGCGTTACCTACTCGACGGGCTCGACATCCTCCCGCTCACCGAACCGGTGATCGGT
CTAGCTGAAACGATCGGACCGGCCACCCTACGTTCTCTCGACGCGATTCACCTCGCGGCCGCAGCCCAGATCAAGCGGGA
ACTGACAGCCTTCGTCACCTACGACCACCGATTGTTGAGCGGATGCCGTGAGGTCGGCTTCGTCACCGCCTCACCCGGCG
CAGTCCGGTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT156718 WP_003156188.1 NZ_CP053376:509033-509314 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT156718 NZ_CP069069:3842544-3842822 [Mycobacterium tuberculosis]
GTGGAGGCAATCGGAATCCGAGAACTAAGACAGCACGCATCGCGATACCTCGCCCGGGTTGAAGCCGGCGAGGAACTTGG
CGTCACCAACAAAGGAAGACTTGTGGCCCGACTCATCCCGGTGCAGGCCGCGGAGCGTTCTCGCGAAGCCCTGATTGAAT
CAGGTGTCCTGATTCCGGCTCGTCGTCCACAAAACCTTCTCGACGTCACCGCCGAACCGGCGCGCGGCCGCAAGCGCACC
CTGTCCGATGTTCTCAACGAAATGCGCGACGAGCAGTGA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5