Detailed information of TA system TA156716

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 148000..148577
• Replicon: plasmid pG32-177
• Accession: NZ_CP053372
• Organism: Proteus terrae subsp. cibarius strain G32

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: HND96_RS19340
• Protein ID: WP_071547961.1
• Coordinates: 148245..148577 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: U5N340
• Locus tag: HND96_RS19335
• Protein ID: WP_023159984.1
• Coordinates: 148000..148245 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HND96_RS19310	144208..144615	-	408	WP_004364961.1	Cd(II)/Pb(II)-responsive transcriptional regulator	-
HND96_RS19315	144711..145226	+	516	Protein_162	sodium:proton antiporter	-
HND96_RS19325	146736..146963	-	228	WP_096864993.1	DNA partition complex ParG	-
HND96_RS19330	147056..147679	-	624	WP_071547962.1	AAA family ATPase	-
HND96_RS19335	148000..148245	+	246	WP_023159984.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HND96_RS19340	148245..148577	+	333	WP_071547961.1	endoribonuclease MazF	Toxin
HND96_RS19345	148737..149529	+	793	Protein_168	IS5 family transposase	-
HND96_RS19350	149597..151090	+	1494	Protein_169	class I SAM-dependent DNA methyltransferase	-
HND96_RS19355	151157..151861	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
HND96_RS19360	151886..153460	+	1575	WP_071538080.1	PAS domain-containing methyl-accepting chemotaxis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / aph(4)-Ia / aac(3)-IVa / aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE / sul1 / floR / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / dfrA1 / lnu(F)	-	1..177911	177911

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-107)

Antitoxin

• SpoVT-AbrB domain (7-49)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12097.88 Da
• Isoelectric Point: 7.0813

>T156716 WP_071547961.1 NZ_CP053372:148245-148577 [Proteus terrae subsp. cibarius]
MVSRYVPDSGDLIWIDFDPVEGHEQGGHRPAVVLSPFAYNNKVGLLLCVPCTTKGKGYPFECEISNERDSYALADQVTCV
DWRARKVTKKGKVEASELAEIKAKAKALIG

Nucleotide

• Length: 333 bp

>T156716 NZ_CP069069:3788609-3788866 [Mycobacterium tuberculosis]
GTGAGAAGCGTCAACTTCGATCCCGATGCCTGGGAGGACTTCTTGTTCTGGCTGGCCGCTGATCGCAAAACGGCCCGTCG
GATCACCCGGTTGATCGGAGAAATTCAGCGTGATCCGTTCAGCGGGATCGGCAAACCCGAGCCGCTCCAAGGTGAGTTGT
CGGGATACTGGTCGCGCCGGATCGACGACGAACACCGGCTGGTGTATCGAGCGGGCGACGACGAAGTCACGATGCTGAAG
GCCCGATACCACTACTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8786.20 Da
• Isoelectric Point: 5.0351

>AT156716 WP_023159984.1 NZ_CP053372:148000-148245 [Proteus terrae subsp. cibarius]
MAQVTVKKWGNSPSVRLPVAIMQKAALSVDDTVEIAVEEGRIIITPVKAVEYSLDNLLAGITPENIHEKVDFGARTGKEL
I

Nucleotide

• Length: 246 bp

>AT156716 NZ_CP069069:3788337-3788612 [Mycobacterium tuberculosis]
ATGAGCATCAGTGCGAGCGAGGCGAGGCAGCGCCTGTTTCCACTCATCGAACAGGTCAATACCGATCACCAGCCGGTGCG
GATCACCTCCCGGGCCGGCGATGCGGTGCTGATGTCCGCCGACGACTACGACGCGTGGCAGGAAACGGTCTATCTGCTGC
GCTCACCGGAGAACGCCAGGCGGTTGATGGAAGCGGTTGCCCGGGATAAGGCTGGGCACTCGGCTTTCACCAAGTCTGTA
GATGAGCTGCGGGAGATGGCCGGCGGCGAGGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A857E7M9