Detailed information of TA system TA156715

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 124604..125181
• Replicon: plasmid pG32-177
• Accession: NZ_CP053372
• Organism: Proteus terrae subsp. cibarius strain G32

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: U5N651
• Locus tag: HND96_RS19220
• Protein ID: WP_023159957.1
• Coordinates: 124849..125181 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: HND96_RS19215
• Protein ID: WP_096864992.1
• Coordinates: 124604..124849 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HND96_RS19180	120357..121403	+	1047	WP_072070749.1	ParB/RepB/Spo0J family partition protein	-
HND96_RS19185	121406..121750	+	345	WP_023159966.1	single-stranded DNA-binding protein	-
HND96_RS19190	121743..122165	+	423	WP_048821775.1	hypothetical protein	-
HND96_RS19195	122175..122567	+	393	WP_109910891.1	hypothetical protein	-
HND96_RS19200	122917..123285	+	369	WP_109910890.1	hypothetical protein	-
HND96_RS19205	123383..123610	-	228	WP_096864993.1	DNA partition complex ParG	-
HND96_RS19210	123711..124334	-	624	WP_109910889.1	AAA family ATPase	-
HND96_RS19215	124604..124849	+	246	WP_096864992.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HND96_RS19220	124849..125181	+	333	WP_023159957.1	endoribonuclease MazF	Toxin
HND96_RS19225	125212..125592	+	381	WP_096864991.1	hypothetical protein	-
HND96_RS19230	125678..125821	-	144	WP_071547955.1	type I toxin-antitoxin system Hok family toxin	-
HND96_RS19235	126186..126983	-	798	WP_048607643.1	hypothetical protein	-
HND96_RS19240	127085..130132	-	3048	WP_094962951.1	Tn3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / aph(4)-Ia / aac(3)-IVa / aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE / sul1 / floR / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / dfrA1 / lnu(F)	-	1..177911	177911

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (9-107)

Antitoxin

• SpoVT-AbrB domain (7-49)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 12097.88 Da
• Isoelectric Point: 7.0813

>T156715 WP_023159957.1 NZ_CP053372:124849-125181 [Proteus terrae subsp. cibarius]
MVSRYVPDSGDLIWIDFDPVEGHEQGGHRPAVVLSPFAYNNKVGLLLCVPCTTKGKGYPFECELSNERDSYALADQVTCV
DWRARKVTKKGKVEASELAEIKAKAKALIG

Nucleotide

• Length: 333 bp

>T156715 NZ_CP069069:c3721208-3720780 [Mycobacterium tuberculosis]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8800.23 Da
• Isoelectric Point: 5.0508

>AT156715 WP_096864992.1 NZ_CP053372:124604-124849 [Proteus terrae subsp. cibarius]
MAQVTVKKWGNSPSVRLPVAIMQKAALSVDDTVEIAVEEGRIIITPVKAVEYSLENLLAGITPENIHEKVDFGARTGKEL
I

Nucleotide

• Length: 246 bp

>AT156715 NZ_CP069069:c3721454-3721212 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Structures

Toxin

• AlphaFold DB: A0A2P1BQ75