Detailed information of TA system TA156708

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 4454206..4454825
• Replicon: chromosome
• Accession: NZ_CP053364
• Organism: Klebsiella pneumoniae strain BA2275

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: R8WYV2
• Locus tag: C5707_RS21930
• Protein ID: WP_002892050.1
• Coordinates: 4454206..4454424 (-)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: C5707_RS21935
• Protein ID: WP_060617716.1
• Coordinates: 4454451..4454825 (-)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5707_RS21895	4450249..4450512	+	264	WP_002892011.1	type B 50S ribosomal protein L31	-
C5707_RS21900	4450512..4450652	+	141	WP_002892018.1	type B 50S ribosomal protein L36	-
C5707_RS21905	4450649..4451347	-	699	WP_002892021.1	GNAT family N-acetyltransferase	-
C5707_RS21910	4451448..4452899	+	1452	WP_002892023.1	PLP-dependent aminotransferase family protein	-
C5707_RS21915	4452874..4453344	-	471	WP_002892026.1	YlaC family protein	-
C5707_RS21920	4453365..4453505	+	141	WP_004147370.1	hypothetical protein	-
C5707_RS21925	4453477..4454043	-	567	WP_002892030.1	maltose O-acetyltransferase	-
C5707_RS21930	4454206..4454424	-	219	WP_002892050.1	hemolysin expression modulator Hha	Toxin
C5707_RS21935	4454451..4454825	-	375	WP_060617716.1	Hha toxicity modulator TomB	Antitoxin
C5707_RS21940	4455311..4458457	-	3147	WP_002892069.1	multidrug efflux RND transporter permease subunit AcrB	-
C5707_RS21945	4458480..4459673	-	1194	WP_002892072.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8628.00 Da
• Isoelectric Point: 8.9008

>T156708 WP_002892050.1 NZ_CP053364:c4454424-4454206 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Nucleotide

• Length: 219 bp

>T156708 NZ_CP069069:c3083208-3082813 [Mycobacterium tuberculosis]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14412.07 Da
• Isoelectric Point: 4.7295

>AT156708 WP_060617716.1 NZ_CP053364:c4454825-4454451 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFDLNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Nucleotide

• Length: 375 bp

>AT156708 NZ_CP069069:c3082787-3082521 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• AlphaFold DB: A0A2P8K6F2