Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3258428..3259053 Replicon chromosome
Accession NZ_CP053364
Organism Klebsiella pneumoniae strain BA2275

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag C5707_RS16205 Protein ID WP_002882817.1
Coordinates 3258670..3259053 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag C5707_RS16200 Protein ID WP_004150355.1
Coordinates 3258428..3258670 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5707_RS16175 3254419..3255018 + 600 WP_004151865.1 glucose-1-phosphatase -
C5707_RS16180 3255012..3255872 + 861 WP_002882807.1 virulence factor BrkB family protein -
C5707_RS16185 3255869..3256306 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
C5707_RS16190 3256351..3257292 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
C5707_RS16195 3257306..3258223 - 918 WP_171910901.1 alpha/beta hydrolase fold domain-containing protein -
C5707_RS16200 3258428..3258670 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
C5707_RS16205 3258670..3259053 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
C5707_RS16210 3259227..3260156 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
C5707_RS16215 3260153..3260788 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
C5707_RS16220 3260785..3261687 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T156704 WP_002882817.1 NZ_CP053364:3258670-3259053 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T156704 NZ_CP069069:c2882828-2882433 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT156704 WP_004150355.1 NZ_CP053364:3258428-3258670 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT156704 NZ_CP069069:c2883070-2882825 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References