Detailed information of TA system TA156703

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 2853659..2854245
• Replicon: chromosome
• Accession: NZ_CP053364
• Organism: Klebsiella pneumoniae strain BA2275

Toxin (Protein)

• Gene name: doc
• Uniprot ID: W8VD46
• Locus tag: C5707_RS14335
• Protein ID: WP_002920800.1
• Coordinates: 2853659..2854027 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A0H3GZM4
• Locus tag: C5707_RS14340
• Protein ID: WP_002920802.1
• Coordinates: 2854024..2854245 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C5707_RS14315	2849162..2850232	-	1071	WP_002920787.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-
C5707_RS14320	2850234..2851079	-	846	WP_002920789.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
C5707_RS14325	2851076..2851963	-	888	WP_002920792.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
C5707_RS14330	2852070..2853386	-	1317	WP_002920796.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
C5707_RS14335	2853659..2854027	-	369	WP_002920800.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
C5707_RS14340	2854024..2854245	-	222	WP_002920802.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
C5707_RS14345	2854409..2855122	-	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
C5707_RS14350	2855124..2855891	-	768	WP_002920803.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
C5707_RS14355	2855888..2857165	-	1278	WP_002920806.1	branched chain amino acid ABC transporter permease LivM	-
C5707_RS14360	2857162..2858088	-	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2848425..2859259	10834

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (13-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-49)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13552.92 Da
• Isoelectric Point: 8.6410

>T156703 WP_002920800.1 NZ_CP053364:c2854027-2853659 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T156703 NZ_CP069069:2881566-2881943 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8181.18 Da
• Isoelectric Point: 4.7769

>AT156703 WP_002920802.1 NZ_CP053364:c2854245-2854024 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQTARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT156703 NZ_CP069069:2881312-2881569 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGACTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GUD1

Antitoxin

• AlphaFold DB: A0A0H3GZM4