Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 39024..39760 Replicon plasmid pEGKP6.1
Accession NZ_CP053359
Organism Klebsiella pneumoniae strain EGKP6

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag HMI21_RS26315 Protein ID WP_003026803.1
Coordinates 39278..39760 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag HMI21_RS26310 Protein ID WP_003026799.1
Coordinates 39024..39290 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HMI21_RS26295 (HMI21_26720) 34317..35522 - 1206 WP_004206572.1 chromate efflux transporter -
HMI21_RS26300 (HMI21_26725) 35698..36276 - 579 WP_040229494.1 recombinase family protein -
HMI21_RS26305 (HMI21_26730) 36435..38727 + 2293 Protein_40 Tn3 family transposase -
HMI21_RS26310 (HMI21_26735) 39024..39290 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
HMI21_RS26315 (HMI21_26740) 39278..39760 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
HMI21_RS26320 (HMI21_26745) 39876..40046 + 171 Protein_43 ISNCY family transposase -
HMI21_RS26325 (HMI21_26750) 40080..43046 - 2967 WP_001138014.1 Tn3-like element TnAs1 family transposase -
HMI21_RS26330 (HMI21_26755) 43050..43610 - 561 WP_001161490.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sul2 / aph(3'')-Ib / aph(6)-Id / catA2 / blaTEM-1B - 1..184664 184664
- inside IScluster/Tn - - 35698..43610 7912


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T156687 WP_003026803.1 NZ_CP053359:39278-39760 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T156687 NZ_CP069069:1769771-1770175 [Mycobacterium tuberculosis]
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCGGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT156687 WP_003026799.1 NZ_CP053359:39024-39290 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT156687 NZ_CP069069:1769562-1769765 [Mycobacterium tuberculosis]
ATGTCGCGCACCAACATCGACATCGATGACGAACTTGCCGCCGAGGTCATGCGCAGGTTCGGTCTGACCACCAAGAGGGC
GGCGGTCGACCTTGCCCTACGACGGTTGGTCGGGTCGCCGTTGAGCCGTGAGTTTCTGCTCGGGCTGGAAGGCGTCGGCT
GGGAAGGCGACCTGGATGACTTGCGAAGCGATCGCCCAGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References