Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4060250..4060869 Replicon chromosome
Accession NZ_CP053358
Organism Klebsiella pneumoniae strain EGKP6

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HMI21_RS20080 Protein ID WP_002892050.1
Coordinates 4060651..4060869 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag HMI21_RS20075 Protein ID WP_002892066.1
Coordinates 4060250..4060624 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HMI21_RS20065 (4055402) 4055402..4056595 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HMI21_RS20070 (4056618) 4056618..4059764 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
HMI21_RS20075 (4060250) 4060250..4060624 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
HMI21_RS20080 (4060651) 4060651..4060869 + 219 WP_002892050.1 HHA domain-containing protein Toxin
HMI21_RS20085 (4061028) 4061028..4061594 + 567 WP_002892030.1 maltose O-acetyltransferase -
HMI21_RS20090 (4061566) 4061566..4061706 - 141 WP_004147370.1 hypothetical protein -
HMI21_RS20095 (4061727) 4061727..4062197 + 471 WP_002892026.1 YlaC family protein -
HMI21_RS20100 (4062172) 4062172..4063623 - 1452 WP_023302231.1 PLP-dependent aminotransferase family protein -
HMI21_RS20105 (4063724) 4063724..4064422 + 699 WP_002892021.1 GNAT family protein -
HMI21_RS20110 (4064419) 4064419..4064559 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
HMI21_RS20115 (4064559) 4064559..4064822 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T156682 WP_002892050.1 NZ_CP053358:4060651-4060869 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T156682 NZ_CP069069:1387821-1388252 [Mycobacterium tuberculosis]
GTGATCATCCCTGACATCAATCTGCTGCTCTACGCGGTCATCACCGGATTCCCGCAGCACCGGCGCGCGCATGCGTGGTG
GCAAGACACCGTCAACGGCCACACCCGTATCGGGCTGACGTATCCGGCGTTGTTCGGGTTCCTACGGATCGCCACCAGTG
CCCGCGTGCTCGCCGCGCCACTGCCAACCGCGGATGCGATCGCCTATGTGCGCGAGTGGCTTTCGCAGCCGAACGTGGAC
CTACTCACGGCGGGTCCGCGCCACCTGGACATCGCGTTGGGCCTGCTCGACAAGCTCGGCACAGCCAGCCACCTAACCAC
CGATGTGCAACTGGCCGCCTACGGCATCGAATACGACGCCGAGATCCATTCCAGTGACACCGACTTTGCCCGATTCGCCG
ATCTGAAGTGGACCGACCCGTTGCGCGAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT156682 WP_002892066.1 NZ_CP053358:4060250-4060624 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT156682 NZ_CP069069:1387564-1387824 [Mycobacterium tuberculosis]
ATGCGCACCACCTTGACGCTCGATGACGACGTCGTCCGGCTGGTCGAAGACGCAGTGCATCGCGAACGCCGCCCGATGAA
GCAGGTCATCAACGATGCGCTGCGCAGAGCGCTGGCGCCGCCGGTGAAACGGCAGGAGCAGTATCGGTTGGAGCCGCATG
AGTCGGCTGTGCGTTCCGGGTTGGATCTGGCCGGCTTCAACAAGTTGGCCGACGAACTGGAGGATGAGGCGCTGCTGGAT
GCCACGCGTCGGGCCCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References