Detailed information of TA system TA156632

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 2604390..2605060
• Replicon: chromosome
• Accession: NZ_CP053346
• Organism: Sphingomonas sp. AP4-R1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HL653_RS12105
• Protein ID: WP_171744733.1
• Coordinates: 2604390..2604809 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HL653_RS12110
• Protein ID: WP_171744734.1
• Coordinates: 2604806..2605060 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HL653_RS12080	2600080..2601190	-	1111	Protein_2386	IS3 family transposase	-
HL653_RS12085	2602157..2602516	+	360	WP_171744730.1	AraC family transcriptional regulator	-
HL653_RS12090	2602534..2602908	-	375	Protein_2388	hypothetical protein	-
HL653_RS12095	2603160..2603435	-	276	WP_171744731.1	HU family DNA-binding protein	-
HL653_RS12100	2603479..2604393	-	915	WP_171744732.1	HEPN domain-containing protein	-
HL653_RS12105	2604390..2604809	-	420	WP_171744733.1	type II toxin-antitoxin system VapC family toxin	Toxin
HL653_RS12110	2604806..2605060	-	255	WP_171744734.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
HL653_RS12115	2605530..2605787	+	258	WP_171744735.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
HL653_RS12120	2605784..2606167	+	384	WP_171744736.1	type II toxin-antitoxin system VapC family toxin	-
HL653_RS12125	2606272..2606766	+	495	WP_171744737.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2540901..2615912	75011

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (5-83)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15534.48 Da
• Isoelectric Point: 4.7820

>T156632 WP_171744733.1 NZ_CP053346:c2604809-2604390 [Sphingomonas sp. AP4-R1]
MIIDTSALVAILYGEDEASSFTQIIQRADICRLSVANYLELTMVIEKQLGLEGTRHAENFVRRAGIQIEHVTVEQGHIAR
QAFFDFGKGRHKAQLNFGDCFAYALAKAVDEPLLFKGNDFIHTDVENALPHSNDPNDET

Nucleotide

• Length: 420 bp

>T156632 NZ_CP069068:2208162-2208539 [Mycobacterium tuberculosis]
GTGCCGCCCCCTGATCCAGCCGCCATGGGTACCTGGAAGTTCTTCCGGGCATCTGTGGATGGCCGGCCGGTATTCAAGAA
GGAGTTCGACAAGCTTCCTGATCAGGCCCGGGCCGCGCTGATCGTGCTAATGCAGCGGTATCTCGTCGGCGACCTCGCCG
CAGGGAGCATCAAACCGATTCGTGGCGACATTCTGGAGTTGCGATGGCATGAGGCGAACAACCACTTCCGGGTACTGTTC
TTCCGCTGGGGCCAGCATCCCGTAGCGCTGACAGCGTTCTACAAGAACCAGCAGAAGACTCCCAAGACGAAGATCGAGAC
GGCCCTGGACCGGCAGAAAATCTGGAAAAGAGCCTTCGGCGACACCCCACCGATCTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9401.85 Da
• Isoelectric Point: 9.8859

>AT156632 WP_171744734.1 NZ_CP053346:c2605060-2604806 [Sphingomonas sp. AP4-R1]
MGATLNIKDPEAHRLALAIARETGEPMTRVVVEALRDRLSRIQRQRERATIAELMAIARRSADLVTRPYPPHGDELYGVD
GLPK

Nucleotide

• Length: 255 bp

>AT156632 NZ_CP069068:2208581-2209030 [Mycobacterium tuberculosis]
ATGAGCATTGACTTCCCTTTGGGTGACGACCTCGCCGGCTATATTGCCGAGGCGATTGCGGCTGATCCCAGCTTCAAAGG
CACTCTCGAAGACGCCGAGGAGGCACGCAGGCTGGTCGATGCGCTGATTGCGCTGCGCAAGCACTGCCAGCTGAGCCAGG
TTGAGGTTGCTAAGCGTATGGGGGTGCGCCAGCCCACCGTGAGCGGTTTCGAGAAGGAACCCAGCGACCCCAAACTGTCT
ACGCTGCAACGTTATGCCCGTGCATTGGACGCCCGGCTGCGGCTGGTGCTCGAAGTTCCCACGCTTCGCGAAGTGCCTAC
GTGGCATCGGCTCTCCTCTTATCGGGGCTCCGCACGGGACCACCAGGTCCGGGTGGGTGCAGACAAGGAAATCCTGATGC
AGACGAACTGGGCCCGCCACATTTCGGTTCGGCAGGTTGAGGTGGCATGA