Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 452274..452910 Replicon chromosome
Accession NZ_CP053315
Organism Bacillus circulans strain GN03

Toxin (Protein)


Gene name mazF Uniprot ID R9C9X9
Locus tag HLK66_RS02275 Protein ID WP_016201246.1
Coordinates 452560..452910 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A0J1I9K1
Locus tag HLK66_RS02270 Protein ID WP_047944180.1
Coordinates 452274..452555 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HLK66_RS02250 448113..448838 - 726 WP_095331017.1 rhomboid family intramembrane serine protease -
HLK66_RS02255 448948..449298 + 351 WP_171509246.1 holo-ACP synthase -
HLK66_RS02260 449617..450627 + 1011 WP_095331013.1 outer membrane lipoprotein carrier protein LolA -
HLK66_RS02265 450780..451937 + 1158 WP_171509247.1 alanine racemase -
HLK66_RS02270 452274..452555 + 282 WP_047944180.1 CopG family ribbon-helix-helix protein Antitoxin
HLK66_RS02275 452560..452910 + 351 WP_016201246.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
HLK66_RS02280 453442..453807 + 366 WP_047944178.1 STAS domain-containing protein -
HLK66_RS02285 453811..454212 + 402 WP_047944177.1 anti-sigma regulatory factor -
HLK66_RS02290 454224..455234 + 1011 WP_047944176.1 PP2C family protein-serine/threonine phosphatase -
HLK66_RS02295 455290..455622 + 333 WP_095331009.1 anti-sigma factor antagonist -
HLK66_RS02300 455619..456089 + 471 WP_047944173.1 anti-sigma B factor RsbW -
HLK66_RS02305 456067..456864 + 798 WP_047944172.1 RNA polymerase sigma factor SigB -
HLK66_RS02310 456861..457457 + 597 WP_095331007.1 protein phosphatase 2C domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13048.11 Da        Isoelectric Point: 4.9105

>T156612 WP_016201246.1 NZ_CP053315:452560-452910 [Bacillus circulans]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIIAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDEEMMEKVDEALQISLGLIEF

Download         Length: 351 bp

>T156612 NZ_CP069068:718652-719047 [Mycobacterium tuberculosis]
ATGGTGATCGACACGTCCGCGCTCGTTGCGATGCTCAGCGACGAGCCAGACGCAGAGCGGTTCGAGGCCGCCGTCGAAGC
CGACCACATCCGGCTGATGTCGACGGCGTCTTACCTGGAAACGGCACTCGTGATAGAAGCCCGCTTCGGTGAACCGGGCG
GACGTGAGCTGGATCTGTGGCTTCATCGCGCCGCGGTCGACCTTGTTGCCGTGCATGCCGACCAAGCGGATGCCGCGCGC
GCCGCCTACCGCACGTACGGCAAGGGAAGGCATCGTGCGGGGCTCAACTACGGCGACTGCTTCTCATACGGCCTCGCCAA
GATCAGCGGCCAGCCACTCCTGTTCAAGGGCGAAGATTTCCAACACACCGACATCGCCACGGTCGCGCTGCCCTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10869.46 Da        Isoelectric Point: 8.4775

>AT156612 WP_047944180.1 NZ_CP053315:452274-452555 [Bacillus circulans]
VSESSTTTEIMIKLPKHLLTELDGFVKQENVNRSEFIYQATKMYLRERKKRHIRESMRRGYMEMAKLNLSIASESFLAEY
EAEHTVERLVSGG

Download         Length: 282 bp

>AT156612 NZ_CP069068:718398-718652 [Mycobacterium tuberculosis]
ATGGCGCTGAGTATCAAGCACCCGGAAGCCGACCGGCTCGCGCGAGCGCTTGCGGCGCGCACCGGCGAGACGTTGACCGA
GGCAGTGGTTACCGCGTTGCGCGAGCGGCTCGCTCGTGAGACTGGGCGTGCCCGTGTTGTCCCGTTGCGCGACGAGCTTG
CCGCGATTCGGCACCGGTGCGCAGCGTTGCCGGTGGTCGACAACCGGTCCGCTGAGGCGATTCTCGGCTATGACGAGCGC
GGATTGCCGGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB R9C9X9

Antitoxin

Source ID Structure
AlphaFold DB A0A0J1I9K1

References