Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 116279..116804 Replicon plasmid pM1-5_120
Accession NZ_CP053298
Organism Escherichia coli strain M1/5

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag HLK60_RS25690 Protein ID WP_001159871.1
Coordinates 116279..116584 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag HLK60_RS25695 Protein ID WP_000813630.1
Coordinates 116586..116804 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HLK60_RS25675 113838..114077 + 240 Protein_132 conjugal transfer protein -
HLK60_RS25685 115472..116278 - 807 WP_021517370.1 site-specific integrase -
HLK60_RS25690 116279..116584 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
HLK60_RS25695 116586..116804 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
HLK60_RS25700 117432..117662 + 231 WP_001261286.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
HLK60_RS25705 117659..118075 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
HLK60_RS25710 118150..119715 + 1566 WP_021517369.1 AAA family ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sitABCD iutA / iucD / iucC / iucB / iucA / senB 1..119964 119964


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T156604 WP_001159871.1 NZ_CP053298:c116584-116279 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T156604 NZ_CP069068:366237-366662 [Mycobacterium tuberculosis]
GTGACTGACCAGCGCTGGCTGATCGACAAGTCGGCGCTGGTGCGGCTCACGGACAGCCCTGACATGGAAATCTGGTCGAA
CCGGATCGAACGCGGCCTGGTACACATCACGGGCGTGACACGCTTGGAAGTAGGGTTCTCGGCCGAATGCGGGGAGATAG
CGCGACGGGAGTTTCGTGAACCGCCGCTGTCTGCGATGCCCGTGGAATACCTAACCCCGAGAATTGAAGACCGTGCGCTC
GAGGTGCAGACCTTGCTTGCCGACCGCGGACACCACCGTGGCCCGTCGATCCCGGATCTGCTCATCGCCGCGACAGCCGA
ACTGTCGGGCTTGACGGTACTGCACGTCGACAAGGACTTTGACGCCATCGCCGCGCTTACCGGTCAGAAAACAGAACGGC
TCACGCATCGCCCGCCTTCCGCTTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT156604 WP_000813630.1 NZ_CP053298:c116804-116586 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT156604 NZ_CP069068:366019-366240 [Mycobacterium tuberculosis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References