Detailed information of TA system TA156603

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 39505..40064
• Replicon: plasmid pM1-5_120
• Accession: NZ_CP053298
• Organism: Escherichia coli strain M1/5

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: HLK60_RS25230
• Protein ID: WP_021517414.1
• Coordinates: 39505..39780 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C1J8K5
• Locus tag: HLK60_RS25235
• Protein ID: WP_001178089.1
• Coordinates: 39780..40064 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HLK60_RS25200	34527..35506	-	980	Protein_37	IS5 family transposase	-
HLK60_RS25205	35608..36120	+	513	WP_021517418.1	autotransporter outer membrane beta-barrel domain-containing protein	-
HLK60_RS25215	37362..37667	+	306	Protein_40	IS91 family transposase	-
HLK60_RS25220	38187..38687	-	501	Protein_41	enterotoxin production-related protein TieB	-
HLK60_RS25225	38745..39442	+	698	Protein_42	IS1 family transposase	-
HLK60_RS25230	39505..39780	-	276	WP_021517414.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HLK60_RS25235	39780..40064	-	285	WP_001178089.1	ribbon-helix-helix domain-containing protein	Antitoxin
HLK60_RS25240	40969..41826	-	858	WP_021517415.1	incFII family plasmid replication initiator RepA	-
HLK60_RS25245	41819..41893	-	75	WP_001375168.1	RepA leader peptide Tap	-
HLK60_RS25250	42129..42386	-	258	WP_000083826.1	replication regulatory protein RepA	-
HLK60_RS25255	42626..43216	-	591	WP_000766805.1	DUF2726 domain-containing protein	-
HLK60_RS25260	43255..43464	-	210	WP_001309247.1	hemolysin expression modulator Hha	-
HLK60_RS25265	43510..43971	-	462	WP_001233842.1	thermonuclease family protein	-
HLK60_RS25270	44216..44428	-	213	WP_001309245.1	ANR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	iutA / iucD / iucC / iucB / iucA / senB	1..119964	119964

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10793.50 Da
• Isoelectric Point: 9.5302

>T156603 WP_021517414.1 NZ_CP053298:c39780-39505 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREIRWIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T156603 NZ_CP069068:365669-365971 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10474.89 Da
• Isoelectric Point: 5.6558

>AT156603 WP_001178089.1 NZ_CP053298:c40064-39780 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT156603 NZ_CP069068:366019-366240 [Mycobacterium tuberculosis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A731IHA8