Detailed information of TA system TA156601

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4570841..4571673
• Replicon: chromosome
• Accession: NZ_CP053296
• Organism: Escherichia coli strain M1/5

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: HLK60_RS22175
• Protein ID: WP_000854753.1
• Coordinates: 4571299..4571673 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NQ68
• Locus tag: HLK60_RS22170
• Protein ID: WP_001540478.1
• Coordinates: 4570841..4571209 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HLK60_RS22135	4566674..4567354	+	681	WP_001282919.1	WYL domain-containing protein	-
HLK60_RS22140	4567502..4568179	+	678	WP_001097301.1	hypothetical protein	-
HLK60_RS22145	4568185..4568418	+	234	WP_001278283.1	DUF905 family protein	-
HLK60_RS22150	4568508..4569326	+	819	WP_001773857.1	DUF945 domain-containing protein	-
HLK60_RS22155	4569417..4569902	+	486	WP_000214398.1	antirestriction protein	-
HLK60_RS22160	4569918..4570394	+	477	WP_001186786.1	RadC family protein	-
HLK60_RS22165	4570457..4570678	+	222	WP_000692311.1	DUF987 domain-containing protein	-
HLK60_RS22170	4570841..4571209	+	369	WP_001540478.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HLK60_RS22175	4571299..4571673	+	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
HLK60_RS22180	4571670..4572158	+	489	WP_000777545.1	hypothetical protein	-
HLK60_RS22185	4572170..4572367	+	198	WP_000839281.1	DUF957 domain-containing protein	-
HLK60_RS22190	4572464..4573033	+	570	WP_001560692.1	DUF4942 domain-containing protein	-
HLK60_RS22195	4573782..4575320	+	1539	WP_001187191.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4546026..4575320	29294

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T156601 WP_000854753.1 NZ_CP053296:4571299-4571673 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T156601 NZ_CP069068:71516-71917 [Mycobacterium tuberculosis]
GTGGATGAATGTGTAGTCGACGCGGCGGCCGTGGTTGACGCTCTCGCCGGCAAGGGCGCCAGCGCGATCGTTCTGCGCGG
TTTGCTCAAGGAGTCGATTTCTAACGCGCCGCATTTGCTGGACGCAGAGGTCGGACATGCACTCCGCCGCGCCGTGCTCA
GCGACGAAATCTCCGAAGAGCAGGCTCGCGCCGCGTTGGATGCCTTGCCTTATCTCATCGACAATCGTTACCCGCACAGC
CCACGACTGATCGAATACACATGGCAGCTAAGGCACAACGTCACGTTCTACGACGCCCTTTACGTCGCACTGGCCACCGC
ACTGGATGTCCCGCTGCTCACGGGCGACTCGCGGCTTGCGGCCGCGCCGGGCCTTCCGTGCGAAATCAAACTCGTTCGGT
GA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13652.53 Da
• Isoelectric Point: 7.8398

>AT156601 WP_001540478.1 NZ_CP053296:4570841-4571209 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT156601 NZ_CP069068:71284-71523 [Mycobacterium tuberculosis]
ATGGCTACCATTCAAGTTCGGGATTTGCCCGAAGATGTCGCCGAAACCTATCGACGGCGCGCCACCGCAGCGGGGCAGTC
GCTGCAGACGTATATGCGCACCAAGCTCATCGAAGGGGTGCGGGGCCGAGACAAGGCCGAGGCAATCGAGATCCTGGAAC
AGGCGCTCGCCAGCACTGCCAGCCCAGGCATCAGCCGGGAGACCATCGAGGCATCCCGGCGGGAGCTCAGGGGTGGATGA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0

Antitoxin

• AlphaFold DB: S1NQ68