Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4179070..4179620 Replicon chromosome
Accession NZ_CP053296
Organism Escherichia coli strain M1/5

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag HLK60_RS20210 Protein ID WP_001160966.1
Coordinates 4179070..4179384 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1NMQ0
Locus tag HLK60_RS20215 Protein ID WP_000125568.1
Coordinates 4179387..4179620 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HLK60_RS20185 4174745..4176031 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
HLK60_RS20190 4176031..4177020 + 990 WP_000241225.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
HLK60_RS20195 4177017..4177838 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
HLK60_RS20200 4177841..4178218 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
HLK60_RS20205 4178225..4179073 + 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
HLK60_RS20210 4179070..4179384 - 315 WP_001160966.1 CcdB family protein Toxin
HLK60_RS20215 4179387..4179620 - 234 WP_000125568.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
HLK60_RS20220 4179706..4180185 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
HLK60_RS20225 4180377..4182239 - 1863 WP_000377109.1 glutathione-regulated potassium-efflux system protein KefC -
HLK60_RS20230 4182232..4182762 - 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
HLK60_RS20235 4182870..4184201 - 1332 WP_001183207.1 MFS transporter -
HLK60_RS20240 4184260..4184547 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T156597 WP_001160966.1 NZ_CP053296:c4179384-4179070 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T156597 NZ_CP069067:c3724291-3723863 [Mycobacterium tuberculosis]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8560.55 Da        Isoelectric Point: 5.8062

>AT156597 WP_000125568.1 NZ_CP053296:c4179620-4179387 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT156597 NZ_CP069067:c3724537-3724295 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A829GEF2

References