Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3703963..3704581 Replicon chromosome
Accession NZ_CP053296
Organism Escherichia coli strain M1/5

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HLK60_RS17970 Protein ID WP_001291435.1
Coordinates 3704363..3704581 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HLK60_RS17965 Protein ID WP_000344800.1
Coordinates 3703963..3704337 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HLK60_RS17955 3699052..3700245 + 1194 WP_001538394.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HLK60_RS17960 3700268..3703417 + 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
HLK60_RS17965 3703963..3704337 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HLK60_RS17970 3704363..3704581 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
HLK60_RS17975 3704754..3705305 + 552 WP_000102539.1 maltose O-acetyltransferase -
HLK60_RS17980 3705421..3705891 + 471 WP_000136192.1 YlaC family protein -
HLK60_RS17985 3706055..3707605 + 1551 WP_001538392.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HLK60_RS17990 3707647..3708000 - 354 WP_000878140.1 DUF1428 domain-containing protein -
HLK60_RS18000 3708379..3708690 + 312 WP_000409908.1 MGMT family protein -
HLK60_RS18005 3708721..3709293 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T156595 WP_001291435.1 NZ_CP053296:3704363-3704581 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T156595 NZ_CP069067:3197263-3197706 [Mycobacterium tuberculosis]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT156595 WP_000344800.1 NZ_CP053296:3703963-3704337 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT156595 NZ_CP069067:3197019-3197276 [Mycobacterium tuberculosis]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References