Detailed information of TA system TA156583

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2057884..2058365
• Replicon: chromosome
• Accession: NZ_CP053296
• Organism: Escherichia coli strain M1/5

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: -
• Locus tag: HLK60_RS09375
• Protein ID: WP_053901079.1
• Coordinates: 2057884..2058174 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: A0A0P7NPJ2
• Locus tag: HLK60_RS09380
• Protein ID: WP_000100895.1
• Coordinates: 2058174..2058365 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HLK60_RS09340	2053149..2053352	-	204	WP_000096346.1	DUF4224 domain-containing protein	-
HLK60_RS09345	2053411..2055885	-	2475	WP_021538049.1	exonuclease	-
HLK60_RS09350	2055981..2056169	-	189	WP_000199480.1	DUF1482 family protein	-
HLK60_RS09355	2056166..2056354	-	189	WP_000449172.1	cell division inhibitor	-
HLK60_RS09360	2056930..2057124	+	195	WP_001295058.1	hypothetical protein	-
HLK60_RS09365	2057113..2057451	-	339	WP_000394541.1	hypothetical protein	-
HLK60_RS09370	2057463..2057615	-	153	WP_021538048.1	DUF1391 family protein	-
HLK60_RS09375	2057884..2058174	-	291	WP_053901079.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HLK60_RS09380	2058174..2058365	-	192	WP_000100895.1	hypothetical protein	Antitoxin
HLK60_RS09385	2058383..2058883	-	501	WP_000824162.1	helix-turn-helix transcriptional regulator	-
HLK60_RS09390	2058991..2059266	+	276	WP_042969575.1	helix-turn-helix domain-containing protein	-
HLK60_RS09395	2059250..2059675	+	426	WP_000693921.1	Rha family transcriptional regulator	-
HLK60_RS09400	2059744..2060781	+	1038	WP_178318134.1	phage replisome organizer	-
HLK60_RS09405	2060693..2061235	+	543	WP_072130322.1	replication protein	-
HLK60_RS09410	2061269..2061985	+	717	WP_100272965.1	DUF1627 domain-containing protein	-
HLK60_RS09415	2062018..2062299	+	282	WP_072146789.1	DNA-binding protein	-
HLK60_RS09420	2062296..2062592	+	297	WP_024188212.1	DUF4406 domain-containing protein	-
HLK60_RS09425	2062589..2063050	+	462	WP_001209475.1	ead/Ea22-like family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11160.01 Da
• Isoelectric Point: 9.9854

>T156583 WP_053901079.1 NZ_CP053296:c2058174-2057884 [Escherichia coli]
MPPILWLPSARDDLRQIVAYIAKENPPAARRLKIRIETSVLSLTEHPYLYPPSERVPSLREIVTHPNYIILYRVTASNIE
IVNVVHSRRQYPNKTC

Nucleotide

• Length: 291 bp

>T156583 NZ_CP069067:c2870129-2869710 [Mycobacterium tuberculosis]
GTGACGGCACTGCTCGACGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7003.80 Da
• Isoelectric Point: 4.0270

>AT156583 WP_000100895.1 NZ_CP053296:c2058365-2058174 [Escherichia coli]
MSTALSPIISEFETVEQENSYNEWLRAKVAASLSDPRPAIPHDEVMAEMENLIAQLAATNRSE

Nucleotide

• Length: 192 bp

>AT156583 NZ_CP069067:c2870350-2870126 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0P7NPJ2