Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1904415..1905246 Replicon chromosome
Accession NZ_CP053296
Organism Escherichia coli strain M1/5

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag HLK60_RS08845 Protein ID WP_000854814.1
Coordinates 1904415..1904789 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID S1PWQ3
Locus tag HLK60_RS08850 Protein ID WP_001285586.1
Coordinates 1904878..1905246 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HLK60_RS08805 1899810..1900976 + 1167 WP_001557064.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
HLK60_RS08810 1901095..1901568 + 474 WP_001105408.1 DNA gyrase inhibitor SbmC -
HLK60_RS08815 1901767..1902825 + 1059 WP_001200891.1 FUSC family protein -
HLK60_RS08820 1902997..1903326 + 330 WP_000450409.1 DUF496 family protein -
HLK60_RS08825 1903427..1903609 - 183 WP_024189425.1 ethanolamine utilization protein -
HLK60_RS08830 1903663..1903809 + 147 Protein_1730 transposase domain-containing protein -
HLK60_RS08835 1904098..1904211 - 114 WP_001161660.1 DUF957 domain-containing protein -
HLK60_RS08840 1904224..1904418 - 195 WP_000988601.1 hypothetical protein -
HLK60_RS08845 1904415..1904789 - 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
HLK60_RS08850 1904878..1905246 - 369 WP_001285586.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
HLK60_RS08855 1905320..1905541 - 222 WP_000692323.1 DUF987 domain-containing protein -
HLK60_RS08860 1905610..1906086 - 477 WP_001351157.1 RadC family protein -
HLK60_RS08865 1906102..1906575 - 474 WP_000855074.1 antirestriction protein -
HLK60_RS08870 1906838..1907659 - 822 WP_001234571.1 DUF945 domain-containing protein -
HLK60_RS08875 1907880..1908290 - 411 WP_000846704.1 hypothetical protein -
HLK60_RS08880 1908306..1908983 - 678 WP_001362823.1 hypothetical protein -
HLK60_RS08885 1909119..1910189 - 1071 WP_000102631.1 patatin-like phospholipase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T156582 WP_000854814.1 NZ_CP053296:c1904789-1904415 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T156582 NZ_CP069067:2823757-2824182 [Mycobacterium tuberculosis]
GTGGCGCTGCTCGACGTCAACGCATTGGTCGCGCTGGCGTGGGACTCACACATCCACCACGCCCGGATCCGCGAGTGGTT
TACCGCCAACGCCACGCTCGGCTGGGCGACTTGCCCGCTCACCGAAGCCGGCTTCGTGCGGGTGTCGACGAACCCAAAAG
TACTTCCCAGCGCGATCGGGATCGCAGACGCTCGACGGGTCCTCGTGGCACTACGCGCCGTGGGAGGCCACCGCTTCCTG
GCTGACGACGTATCGCTCGTCGATGACGATGTTCCGTTGATCGTCGGTTATCGCCAGGTGACCGACGCCCATCTGCTGAC
ACTCGCCCGCCGGCGCGGCGTCCGCCTGGTCACCTTCGACGCCGGTGTCTTCACCCTCGCCCAACAACGCCCCAAGACGC
CAGTGGAGCTGCTGACCATCCTCTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13563.41 Da        Isoelectric Point: 5.0468

>AT156582 WP_001285586.1 NZ_CP053296:c1905246-1904878 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELSPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT156582 NZ_CP069067:2823530-2823751 [Mycobacterium tuberculosis]
GTGCGAACGACACTTGACCTTGACGACGATGTGATCGCCGCGGCACGTGAACTTGCCTCCAGCCAGCGCCGCTCGCTCGG
CTCGGTGATTTCCGAACTCGCACGCCGTGGTCTCATGCCCGGACGCGTCGAGGCTGACGACGGGCTGCCGGTGATCCGCG
TTCCAGCCGGGACCCCGCCGATCACACCGGAGATGGTCCGTCGCGCGCTCGATAAGGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB S1PWQ3

References