Detailed information of TA system TA156579

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 841516..842350
• Replicon: chromosome
• Accession: NZ_CP053296
• Organism: Escherichia coli strain M1/5

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PLF5
• Locus tag: HLK60_RS03985
• Protein ID: WP_000854690.1
• Coordinates: 841516..841893 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1P7N8
• Locus tag: HLK60_RS03990
• Protein ID: WP_001305076.1
• Coordinates: 841982..842350 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HLK60_RS03955	837910..838065	-	156	WP_000729638.1	hypothetical protein	-
HLK60_RS03960	838497..839430	-	934	Protein_778	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
HLK60_RS03965	839423..839818	-	396	WP_000208382.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
HLK60_RS03970	839887..840732	-	846	WP_001529401.1	DUF4942 domain-containing protein	-
HLK60_RS03975	840817..841014	-	198	WP_000839293.1	DUF957 domain-containing protein	-
HLK60_RS03980	841031..841519	-	489	WP_000761699.1	hypothetical protein	-
HLK60_RS03985	841516..841893	-	378	WP_000854690.1	TA system toxin CbtA family protein	Toxin
HLK60_RS03990	841982..842350	-	369	WP_001305076.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HLK60_RS03995	842400..843044	-	645	WP_000094916.1	hypothetical protein	-
HLK60_RS04000	843063..843284	-	222	WP_000692329.1	DUF987 domain-containing protein	-
HLK60_RS04005	843347..843823	-	477	WP_001186726.1	RadC family protein	-
HLK60_RS04010	843839..844324	-	486	WP_000849565.1	antirestriction protein	-
HLK60_RS04015	844379..845197	-	819	WP_001234620.1	DUF945 domain-containing protein	-
HLK60_RS04020	845298..845531	-	234	WP_000902034.1	DUF905 family protein	-
HLK60_RS04025	845610..846065	-	456	WP_000581502.1	hypothetical protein	-
HLK60_RS04030	846141..847268	-	1128	Protein_792	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	837910..838065	155

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14057.04 Da
• Isoelectric Point: 9.1510

>T156579 WP_000854690.1 NZ_CP053296:c841893-841516 [Escherichia coli]
MKTLPDTHVRAASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYAQVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T156579 NZ_CP069067:2340990-2341400 [Mycobacterium tuberculosis]
TTGGCTGAGCCACGGCGAGGAGACCTTTGGCTGGTCAGCCTCGGCGCCGCTCGCGCGGGTGAGCCCGGCAAGCATCGGCC
CGCGGTGGTCGTTTCCGTGGACGAGCTACTCACCGGAATCGACGACGAACTCGTTGTCGTCGTGCCGGTGTCAAGCTCGC
GCTCCCGCACCCCACTCCGGCCACCTGTCGCGCCCTCAGAAGGTGTAGCTGCCGATAGCGTCGCGGTGTGCCGCGGCGTC
CGCGCGGTCGCTCGTGCCCGACTCGTGGAGCGACTCGGCGCCCTCAAACCCGCCACGATGCGCGCAATCGAAAACGCCCT
GACCCTGATCCTCGGCCTCCCGACGGGACCTGAGCGCGGCGAGGCGGCGACCCATTCTCCCGTACGGTGGACGGGTGGCC
GGGACCCGTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13560.39 Da
• Isoelectric Point: 4.7830

>AT156579 WP_001305076.1 NZ_CP053296:c842350-841982 [Escherichia coli]
MSDTLPGTTLPDDNKDLPWWGLPCTVTPCFGACLVQEGNRLHYLADRAGIRGRFSDADAYHPDQAFPLLMKQPELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT156579 NZ_CP069067:2340764-2340997 [Mycobacterium tuberculosis]
ATGTCTACATCCACGACGATTAGGGTTTCAACCCAGACTCGGGATCGTCTGGCCGCCCAAGCCCGCGAACGGGGAATCTC
GATGTCGGCTCTGCTCACCGAACTGGCCGCCCAGGCCGAGCGCCAGGCAATCTTCCGCGCCGAACGCGAGGCCTCGCACG
CCGAGACGACCACCCAGGCAGTCCGCGACGAGGACCGCGAGTGGGAGGGCACGGTAGGCGACGGCCTTGGCTGA

Structures

Toxin

• AlphaFold DB: S1PLF5

Antitoxin

• AlphaFold DB: S1P7N8