Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 5122552..5123194 Replicon chromosome
Accession NZ_CP053289
Organism Bacillus cereus strain WPySW2

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag HLB41_RS26030 Protein ID WP_000635963.1
Coordinates 5122552..5122902 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag HLB41_RS26035 Protein ID WP_000004570.1
Coordinates 5122907..5123194 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HLB41_RS26015 5119488..5119946 - 459 WP_000344234.1 SprT family protein -
HLB41_RS26020 5120142..5120258 + 117 WP_001143642.1 cortex morphogenetic protein CmpA -
HLB41_RS26025 5120316..5122484 - 2169 WP_000426242.1 RNA-binding transcriptional accessory protein -
HLB41_RS26030 5122552..5122902 - 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
HLB41_RS26035 5122907..5123194 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
HLB41_RS26040 5123503..5124672 - 1170 WP_000390615.1 alanine racemase -
HLB41_RS26045 5124790..5125740 - 951 WP_002004297.1 outer membrane lipoprotein carrier protein LolA -
HLB41_RS26050 5125897..5126256 - 360 WP_000583416.1 holo-ACP synthase -
HLB41_RS26055 5126350..5126922 + 573 WP_000908523.1 rhomboid family intramembrane serine protease -
HLB41_RS26060 5126915..5127877 - 963 WP_000961165.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T156575 WP_000635963.1 NZ_CP053289:c5122902-5122552 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T156575 NZ_CP069067:c2244077-2243658 [Mycobacterium tuberculosis]
ATGATCGTGGACACAAGCGCCGTGGTGGCCCTGGTTCAAGGCGAGCGGCCGCACGCCACCCTGGTCGCGGCCGCCCTGGC
CGGCGCCCATAGCCCCGTCATGTCTGCACCCACCGTCGCCGAATGCCTGATTGTCTTGACCGCCCGTCACGGCCCCGTTG
CGCGCACGATCTTCGAACGACTTCGCAGCGAAATCGGCTTGAGCGTGTCATCTTTCACCGCCGAGCATGCCGCTGCCACG
CAACGAGCCTTTCTGCGATACGGCAAGGGGCGCCACCGCGCGGCTCTCAACTTCGGAGACTGTATGACGTACGCGACCGC
CCAGCTGGGCCACCAACCACTGCTGGCCGTCGGCAACGACTTCCCGCAAACCGACCTTGAGTTCCGCGGCGTCGTCGGCT
ACTGGCCAGGCGTCGCGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT156575 WP_000004570.1 NZ_CP053289:c5123194-5122907 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT156575 NZ_CP069067:c2244346-2244086 [Mycobacterium tuberculosis]
GTGGCGCTGAATATCAAAGACCCTGAGGTAGACCGACTAGCCGCCGAACTCGCTGACCGGCTGCACACCAGCAAGACTGC
CGCCATCCGGCATGCCCTGTCTGCCCAGCTGGCGTTTTTGGAGTCGCGCGCCGGCGACCGTGAGGCACAACTTCTCGACA
TCTTGCGTACCGAAATCTGGCCCCTGCTTGCCGACCGCTCCCCCATCACCAAGCTCGAGCGCGAACAAATCCTCGGCTAC
GACCCCGCAACCGGAGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References