Detailed information of TA system TA156574

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 3017685..3018663
• Replicon: chromosome
• Accession: NZ_CP053289
• Organism: Bacillus cereus strain WPySW2

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: HLB41_RS15280
• Protein ID: WP_000624975.1
• Coordinates: 3017926..3018663 (-)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: W8YZL5
• Locus tag: HLB41_RS15275
• Protein ID: WP_000237818.1
• Coordinates: 3017685..3017813 (-)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HLB41_RS15245	3013169..3013420	+	252	WP_000827560.1	response regulator transcription factor	-
HLB41_RS15250	3013663..3014265	-	603	WP_065382625.1	hypothetical protein	-
HLB41_RS15255	3014291..3015100	-	810	WP_065382626.1	C39 family peptidase	-
HLB41_RS15260	3015346..3016815	-	1470	WP_065382524.1	beta-Ala-His dipeptidase	-
HLB41_RS15265	3017027..3017416	+	390	WP_000713862.1	YxeA family protein	-
HLB41_RS15270	3017436..3017612	-	177	WP_065382523.1	stage II sporulation protein SB	-
HLB41_RS15275	3017685..3017813	-	129	WP_000237818.1	hypothetical protein	Antitoxin
HLB41_RS15280	3017926..3018663	-	738	WP_000624975.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
HLB41_RS15285	3018902..3019639	-	738	WP_000594152.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
HLB41_RS15290	3019806..3020288	-	483	WP_072770281.1	MarR family winged helix-turn-helix transcriptional regulator	-
HLB41_RS15295	3020405..3022081	+	1677	WP_139256968.1	alpha-keto acid decarboxylase family protein	-
HLB41_RS15300	3022239..3023021	-	783	Protein_3013	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28278.98 Da
• Isoelectric Point: 8.2998

>T156574 WP_000624975.1 NZ_CP053289:c3018663-3017926 [Bacillus cereus]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMAIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T156574 NZ_CP069067:c2223671-2223375 [Mycobacterium tuberculosis]
GTGAGTAGCCGATACCTTCTCTCGCCTGCCGCGCAGGCACATCTGGAAGAGATCTGGGACTGCACCTATGACCGTTGGGG
TGTCGATCAGGCCGAGCAGTACCTGCGCGAACTTCAACACGCTATCGACCGTGCTGCGGCAAACCCGCGGATCGGACGAG
CGTGCGACGAGATTCGCCCCGGCTATCGCAAGCTCTCGGCCGGGTCACACACGTTGTTCTATCGGGTGACTGGCGAAGGC
ACCATCGACGTCGTGCGAGTCCTGCACCAACGGATGGACGTCGACCGGAACCTCTGA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 5011.40 Da
• Isoelectric Point: 3.9150

>AT156574 WP_000237818.1 NZ_CP053289:c3017813-3017685 [Bacillus cereus]
VVKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT156574 NZ_CP069067:c2223919-2223668 [Mycobacterium tuberculosis]
GTGGGTAAGAACACGTCCTTCGTCCTCGACGAGCACTACAGCGCCTTCATCGACGGCGAGATCGCCGCGGGCCGCTACCG
GTCGGCCAGTGAAGTCATCCGCTCCGCGTTGCGACTGCTCGAGGACCGTGAAACCCAGCTGCGCGCGCTTCGTGAGGCTC
TCGAGGCCGGCGAACGCAGCGGCAGCTCGACACCGTTCGACTTCGACGGATTCCTCGGTCGTAAGCGGGCTGACGCCTCG
CGTGGCCGGTGA

Structures

Antitoxin

• AlphaFold DB: W8YZL5