Detailed information of TA system TA156496

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1386189..1386814
• Replicon: chromosome
• Accession: NZ_CP053281
• Organism: Escherichia coli strain SCU-308

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HHJ45_RS06760
• Protein ID: WP_000911330.1
• Coordinates: 1386416..1386814 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: HHJ45_RS06755
• Protein ID: WP_000450524.1
• Coordinates: 1386189..1386416 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HHJ45_RS06730	1381992..1382462	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
HHJ45_RS06735	1382462..1383034	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
HHJ45_RS06740	1383180..1384058	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
HHJ45_RS06745	1384075..1385109	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
HHJ45_RS06750	1385322..1386035	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
HHJ45_RS06755	1386189..1386416	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
HHJ45_RS06760	1386416..1386814	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
HHJ45_RS06765	1386961..1387824	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
HHJ45_RS06770	1387839..1389854	+	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
HHJ45_RS06775	1389928..1390626	+	699	WP_000679823.1	esterase	-
HHJ45_RS06780	1390736..1390936	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T156496 WP_000911330.1 NZ_CP053281:1386416-1386814 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T156496 NZ_CP069066:714451-714846 [Mycobacterium tuberculosis]
ATGGTGATCGACACGTCCGCGCTCGTTGCGATGCTCAGCGACGAGCCAGACGCAGAGCGGTTCGAGGCCGCCGTCGAAGC
CGACCACATCCGGCTGATGTCGACGGCGTCTTACCTGGAAACGGCACTCGTGATAGAAGCCCGCTTCGGTGAACCGGGCG
GACGTGAGCTGGATCTGTGGCTTCATCGCGCCGCGGTCGACCTTGTTGCCGTGCATGCCGACCAAGCGGATGCCGCGCGC
GCCGCCTACCGCACGTACGGCAAGGGAAGGCATCGTGCGGGGCTCAACTACGGCGACTGCTTCTCATACGGCCTCGCCAA
GATCAGCGGCCAGCCACTCCTGTTCAAGGGCGAAGATTTCCAACACACCGACATCGCCACGGTCGCGCTGCCCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT156496 WP_000450524.1 NZ_CP053281:1386189-1386416 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT156496 NZ_CP069066:714197-714451 [Mycobacterium tuberculosis]
ATGGCGCTGAGTATCAAGCACCCGGAAGCCGACCGGCTCGCGCGAGCGCTTGCGGCGCGCACCGGCGAGACGTTGACCGA
GGCAGTGGTTACCGCGTTGCGCGAGCGGCTCGCTCGTGAGACTGGGCGTGCCCGTGTTGTCCCGTTGCGCGACGAGCTTG
CCGCGATTCGGCACCGGTGCGCAGCGTTGCCGGTGGTCGACAACCGGTCCGCTGAGGCGATTCTCGGCTATGACGAGCGC
GGATTGCCGGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33