Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4227027..4227524 Replicon chromosome
Accession NZ_CP053259
Organism Escherichia coli strain GF3-3

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HLY12_RS19950 Protein ID WP_001173343.1
Coordinates 4227027..4227200 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HLY12_RS19955 Protein ID WP_000398619.1
Coordinates 4227228..4227524 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HLY12_RS19940 4223697..4225154 + 1458 WP_000856824.1 dipeptide/tripeptide permease DtpC -
HLY12_RS19945 4225391..4226908 + 1518 WP_001295074.1 lysine--tRNA ligase -
HLY12_RS19950 4227027..4227200 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HLY12_RS19955 4227228..4227524 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HLY12_RS19960 4227751..4228023 - 273 WP_000405647.1 N-acetyltransferase -
HLY12_RS19965 4228035..4228265 - 231 WP_000371704.1 (4Fe-4S)-binding protein -
HLY12_RS19970 4228446..4230077 + 1632 WP_001216470.1 sensor histidine kinase -
HLY12_RS19975 4230074..4230793 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T156490 WP_001173343.1 NZ_CP053259:c4227200-4227027 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T156490 NZ_CP069066:c442186-441593 [Mycobacterium tuberculosis]
GTGAAGCGGCTCGATCTGGTCGCCGGGCCCAACGGCGCCGGCAAGTCGACGTTCGTCGCCCTCACGCTGGCGCCCTTGCT
GCCCGGCATCGTCTTCGTCAACGCCGACGAAATCGCCAAACAACGCTGGCCCGACGACCCAACATCGCACGCCTACCAGG
CGGCGCAGGTCGCCGCCGACACCCGCGCGAGGCTCATCGACTTGGGCCGGCCGTTCATTGCCGAGACGGTGTTCTCGCAC
CCATCGAAGCTCGAGCTCATCCGCACCGCGCGCACGGCCGGCTACACCGTCGTACTGCACGTGTTGGTTATCCCCGAAGG
CCTGGCGGTCGAGCGCGTCAGGCATCGCGTCGCCGCGGGCGGCCACGATGTGCCGGAGACCAAGATCCGCGAGCGTCACC
GCCGGCTGGCCGAGCTTGTCGCCCAGGCCATCACGCTGGCCGACGGCGCCACCGTCTATGACAACAGCCGGCTCGCGGGC
CCGCGGATCGTCGCTCAGTTCAGCGGGGGTGGCATCATCGGGCGCGCATGCTGGCCTTCGTGGACGCCGCCACCCTTGAT
GTCACGCTGGAGTAACAGGCCTGAGACGGCGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT156490 WP_000398619.1 NZ_CP053259:c4227524-4227228 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT156490 NZ_CP069066:c442604-442215 [Mycobacterium tuberculosis]
GTGCCCAAAGCCGTCGACCGCGTCACGCGCGTCGCCGCGGATCTGGTGGACAGCGCGGCGGCCGAGGGTGCTCGTCAGAG
CCGTTCGGCCAAGCAGCAGCTCGACCACTGGGCCCGCGTCGGTCGGGCGGTGTCGAACCAGCACACGGCGTCGCGGCGCC
GCGTGGAAGCTGCGCTCGCGGGGCATCTGCCGATGACGGACCTGACGCTGGAGGAGGGCGTGGTGTTCAACGCCGAGATC
TCCGCAGCTATCGAGGAGCGCCTGTCGCGAACCAACTACGGCGACGTGCTCGCCGCGCAGGGCATCACCACGGTCGCCCT
GAACGACGCGGGCGACATCGTCGAGCACCGGCCAGACGGCACCTCGGTCGTGCTGGCGGCCACGCCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References