Detailed information of TA system TA156489

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4020435..4021011
• Replicon: chromosome
• Accession: NZ_CP053259
• Organism: Escherichia coli strain GF3-3

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: HLY12_RS18985
• Protein ID: WP_001672355.1
• Coordinates: 4020724..4021011 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A829G8Z0
• Locus tag: HLY12_RS18980
• Protein ID: WP_000063156.1
• Coordinates: 4020435..4020737 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HLY12_RS18960	4016126..4016638	+	513	WP_001175455.1	4-hydroxyphenylacetate 3-monooxygenase reductase subunit	-
HLY12_RS18965	4017031..4019181	+	2151	WP_001298933.1	pyruvate/proton symporter BtsT	-
HLY12_RS18970	4019231..4019434	+	204	WP_000467859.1	YbdD/YjiX family protein	-
HLY12_RS18975	4019445..4020401	+	957	WP_171284470.1	GTPase	-
HLY12_RS18980	4020435..4020737	-	303	WP_000063156.1	BrnA antitoxin family protein	Antitoxin
HLY12_RS18985	4020724..4021011	-	288	WP_001672355.1	BrnT family toxin	Toxin
HLY12_RS18990	4021208..4022374	+	1167	WP_001672354.1	restriction endonuclease	-
HLY12_RS18995	4022438..4024057	+	1620	WP_112924895.1	type I restriction-modification system subunit M	-
HLY12_RS19000	4024047..4025453	+	1407	WP_115444372.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11195.66 Da
• Isoelectric Point: 7.4020

>T156489 WP_001672355.1 NZ_CP053259:c4021011-4020724 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGVVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T156489 NZ_CP069066:361394-361819 [Mycobacterium tuberculosis]
GTGACTGACCAGCGCTGGCTGATCGACAAGTCGGCGCTGGTGCGGCTCACGGACAGCCCTGACATGGAAATCTGGTCGAA
CCGGATCGAACGCGGCCTAGTACACATCACGGGCGTGACACGCTTGGAAGTAGGGTTCTCGGCCGAATGCGGGGAGATAG
CGCGACGGGAGTTTCGTGAACCGCCGCTGTCTGCGATGCCCGTGGAATACCTAACCCCGAGAATTGAAGACCGTGCGCTC
GAGGTGCAGACCTTGCTTGCCGACCGCGGACACCACCGTGGCCCGTCGATCCCGGATCTGCTCATCGCCGCGACAGCCGA
ACTGTCGGGCTTGACGGTACTGCACGTCGACAAGGACTTTGACGCCATCGCCGCGCTTACCGGTCAGAAAACAGAACGGC
TCACGCATCGCCCGCCTTCCGCTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11521.99 Da
• Isoelectric Point: 9.4646

>AT156489 WP_000063156.1 NZ_CP053259:c4020737-4020435 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Nucleotide

• Length: 303 bp

>AT156489 NZ_CP069066:361176-361397 [Mycobacterium tuberculosis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A829G8Z0