Detailed information of TA system TA156487

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2400298..2400936
• Replicon: chromosome
• Accession: NZ_CP053259
• Organism: Escherichia coli strain GF3-3

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A3T4J978
• Locus tag: HLY12_RS11355
• Protein ID: WP_001612987.1
• Coordinates: 2400760..2400936 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: HLY12_RS11350
• Protein ID: WP_001270286.1
• Coordinates: 2400298..2400714 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HLY12_RS11330	2395450..2396391	-	942	WP_171284261.1	ABC transporter permease	-
HLY12_RS11335	2396392..2397405	-	1014	WP_000220411.1	ABC transporter ATP-binding protein	-
HLY12_RS11340	2397423..2398568	-	1146	WP_001612991.1	ABC transporter substrate-binding protein	-
HLY12_RS11345	2398813..2400219	-	1407	WP_069913499.1	PLP-dependent aminotransferase family protein	-
HLY12_RS11350	2400298..2400714	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
HLY12_RS11355	2400760..2400936	-	177	WP_001612987.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
HLY12_RS11360	2401158..2401388	+	231	WP_171284262.1	YncJ family protein	-
HLY12_RS11365	2401480..2403441	-	1962	WP_171284263.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
HLY12_RS11370	2403514..2404050	-	537	WP_000429147.1	DNA-binding transcriptional regulator SutR	-
HLY12_RS11375	2404103..2405317	+	1215	WP_171284264.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2405357..2406505	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6725.78 Da
• Isoelectric Point: 10.9223

>T156487 WP_001612987.1 NZ_CP053259:c2400936-2400760 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKVRFHGMRSVMPRHPCDEIKEPLRKAIQKQLGLS

Nucleotide

• Length: 177 bp

>T156487 NZ_CP069066:360826-361128 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT156487 WP_001270286.1 NZ_CP053259:c2400714-2400298 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT156487 NZ_CP069066:360602-360829 [Mycobacterium tuberculosis]
ATGACGAAAGAGAAGATCTCCGTGACGGTGGACGCGGCCGTCCTCGCGGCGATCGACGCGGACGCCAGGGCGGCGGGTTT
GAATCGGTCGGAAATGATTGAGCAGGCACTGCGCAACGAGCACCTGCGTGTCGCTCTGCGCGATTACACGGCTAAAACCG
TACCGGCGTTGGACATCGATGCCTACGCACAGCGGGTGTACCAGGCGAACCGGGCGGCCGGAAGTTGA

Structures

Toxin

• AlphaFold DB: A0A3T4J978