Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 851370..852024 Replicon chromosome
Accession NZ_CP053259
Organism Escherichia coli strain GF3-3

Toxin (Protein)


Gene name cptA Uniprot ID -
Locus tag HLY12_RS04060 Protein ID WP_001240154.1
Coordinates 851617..852024 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HLY12_RS04055 Protein ID WP_000354046.1
Coordinates 851370..851636 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HLY12_RS04035 847339..848772 - 1434 WP_171284027.1 6-phospho-beta-glucosidase BglA -
HLY12_RS04040 848817..849128 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
HLY12_RS04045 849292..849951 + 660 WP_171284028.1 hemolysin III family protein -
HLY12_RS04050 850147..851127 - 981 WP_001613766.1 tRNA-modifying protein YgfZ -
HLY12_RS04055 851370..851636 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HLY12_RS04060 851617..852024 + 408 WP_001240154.1 protein YgfX Toxin
HLY12_RS04065 852064..852585 - 522 WP_001613762.1 flavodoxin FldB -
HLY12_RS04070 852697..853593 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HLY12_RS04075 853618..854328 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HLY12_RS04080 854334..856067 + 1734 WP_000813202.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(2-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16033.93 Da        Isoelectric Point: 11.5202

>T156476 WP_001240154.1 NZ_CP053259:851617-852024 [Escherichia coli]
VALWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T156476 NZ_CP069065:c3083274-3082918 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT156476 WP_000354046.1 NZ_CP053259:851370-851636 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT156476 NZ_CP069065:c3083488-3083258 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References