Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 274496..275127 Replicon chromosome
Accession NZ_CP053259
Organism Escherichia coli strain GF3-3

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag HLY12_RS01155 Protein ID WP_001259385.1
Coordinates 274496..274771 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID Q31VE4
Locus tag HLY12_RS01160 Protein ID WP_000593554.1
Coordinates 274768..275127 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HLY12_RS01140 269500..270567 + 1068 WP_000361491.1 HlyD family efflux transporter periplasmic adaptor subunit -
HLY12_RS01145 270564..273299 + 2736 WP_001614021.1 ribosome-associated ATPase/putative transporter RbbA -
HLY12_RS01150 273299..274423 + 1125 WP_001314210.1 ABC transporter permease -
HLY12_RS01155 274496..274771 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
HLY12_RS01160 274768..275127 + 360 WP_000593554.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
HLY12_RS01165 275177..276037 - 861 WP_001131788.1 class II aldolase -
HLY12_RS01170 276069..276338 - 270 WP_171283955.1 HPr family phosphocarrier protein -
HLY12_RS01175 276328..277836 - 1509 WP_001298718.1 carbohydrate kinase -
HLY12_RS01180 277829..279187 - 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
HLY12_RS01185 279264..279545 - 282 WP_000084022.1 PTS sugar transporter subunit IIB -
HLY12_RS01190 279542..280015 - 474 WP_001161553.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 265508..280786 15278


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T156473 WP_001259385.1 NZ_CP053259:274496-274771 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T156473 NZ_CP069065:2914077-2914517 [Mycobacterium tuberculosis]
ATGCTGCTCTGTGACACCAACATCTGGCTGGCGTTGGCGCTTTCCGGACACGTGCACCACAGGGCCTCGCGCGCATGGCT
AGACACCATCAACGCGCCCGGAGTCATCCACTTTTGCCGCGCAACCCAACAGTCGCTCCTTCGGCTGTTGACGAATCGGA
CGGTGCTGGGCGCGTATGGCAGCCCACCACTGACCAACCGCGAAGCGTGGGCGGCCTATGCCGCGTTCCTGGATGACGAC
CGCATCGTGCTGGCCGGCGCCGAACCTGATGGTTTGGAGGCCCAGTGGAGAGCCTTCGCCGTTCGCCAGTCGCCGGCGCC
CAAGGTTTGGATGGATGCCTACCTAGCTGCTTTCGCACTTACCGGTGGATTCGAGTTGGTGACGACTGACACCGCCTTCA
CCCAGTACGGCGGAATCGAGCTGCGGCTCCTGGCCAAGTGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13359.25 Da        Isoelectric Point: 4.8838

>AT156473 WP_000593554.1 NZ_CP053259:274768-275127 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT156473 NZ_CP069065:2913803-2914090 [Mycobacterium tuberculosis]
ATGAAGACCACGCTCGACCTGCCTGATGAACTGATGCGCGCTATCAAGGTCCGCGCGGCGCAGCAGGGCCGCAAGATGAA
AGATGTCGTGACCGAACTGCTCAGATCCGGTCTGTCCCAGACGCACAGCGGGGCTCCAATCCCAACGCCGCGGCGCGTGC
AGCTTCCCCTGGTGCATTGCGGTGGCGCGGCTACCCGCGAACAAGAAATGACGCCGGAGCGTGTTGCCGCGGCCTTGCTC
GACCAGGAGGCCCAGTGGTGGTCCGGACACGACGATGCTGCTCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A6H2GM94

References