Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3953264..3953918 Replicon chromosome
Accession NZ_CP053258
Organism Escherichia coli strain GF4-3

Toxin (Protein)


Gene name cptA Uniprot ID -
Locus tag HLY11_RS18525 Protein ID WP_171460405.1
Coordinates 3953511..3953918 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HLY11_RS18520 Protein ID WP_000354046.1
Coordinates 3953264..3953530 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HLY11_RS18500 3949352..3950785 - 1434 WP_171460404.1 6-phospho-beta-glucosidase BglA -
HLY11_RS18505 3950830..3951141 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
HLY11_RS18510 3951305..3951964 + 660 WP_000250274.1 hemolysin III family protein -
HLY11_RS18515 3952041..3953021 - 981 WP_000886074.1 tRNA-modifying protein YgfZ -
HLY11_RS18520 3953264..3953530 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HLY11_RS18525 3953511..3953918 + 408 WP_171460405.1 protein YgfX Toxin
HLY11_RS18530 3953958..3954479 - 522 WP_001055874.1 flavodoxin FldB -
HLY11_RS18535 3954591..3955487 + 897 WP_097751990.1 site-specific tyrosine recombinase XerD -
HLY11_RS18540 3955512..3956222 + 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HLY11_RS18545 3956228..3957961 + 1734 WP_000813188.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16067.00 Da        Isoelectric Point: 11.2669

>T156472 WP_171460405.1 NZ_CP053258:3953511-3953918 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLFAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLLSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T156472 NZ_CP069065:c2853859-2853464 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT156472 WP_000354046.1 NZ_CP053258:3953264-3953530 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT156472 NZ_CP069065:c2854101-2853856 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References