Detailed information of TA system TA156470

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 3403010..3403764
• Replicon: chromosome
• Accession: NZ_CP053258
• Organism: Escherichia coli strain GF4-3

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: HLY11_RS15755
• Protein ID: WP_171460239.1
• Coordinates: 3403010..3403495 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: HLY11_RS15760
• Protein ID: WP_000801914.1
• Coordinates: 3403486..3403764 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HLY11_RS15735	3398177..3398935	+	759	WP_000815097.1	DeoR/GlpR family transcriptional regulator	-
HLY11_RS15740	3398917..3400515	-	1599	WP_171460237.1	DNA-binding transcriptional regulator RtcR	-
HLY11_RS15745	3400702..3401928	+	1227	WP_171460238.1	RNA-splicing ligase RtcB	-
HLY11_RS15750	3401932..3402960	+	1029	WP_171460690.1	RNA 3'-terminal phosphate cyclase	-
HLY11_RS15755	3403010..3403495	-	486	WP_171460239.1	GNAT family N-acetyltransferase	Toxin
HLY11_RS15760	3403486..3403764	-	279	WP_000801914.1	DUF1778 domain-containing protein	Antitoxin
HLY11_RS15765	3403831..3406536	-	2706	WP_000906970.1	HTH-type transcriptional regulator MalT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (44-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-92)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17764.67 Da
• Isoelectric Point: 9.1189

>T156470 WP_171460239.1 NZ_CP053258:c3403495-3403010 [Escherichia coli]
MGITPPTPLTSEHNLADFCCSDHGMNEWLKKKELKNHSSGLSRVYVICIANTRQVIGYYCLSTGSIQRNLTPGAMRRNAP
ESLPVVVLGRLAIDQAWAGKGLGVALLKDAVYRTMSIAQQVGVRALIVHALDDSVRNFYLKYAFVPSPFQSLTLLYPITL
E

Nucleotide

• Length: 486 bp

>T156470 NZ_CP069065:2851891-2852304 [Mycobacterium tuberculosis]
ATGGTGTTCTGCGTCGACACCAGCGCGTGGCATCACGCGGCGCGGCCGGAAGTTGCGCGCCGATGGTTGGCGGCCTTGTC
CGCGGACCAGATCGGCATCTGCGACCACGTGCGGTTGGAGATCCTGTACTCGGCGAACTCCGCTACCGACTACGACGCGC
TCGCCGACGAACTCGACGGCTTGGCCCGTATACCAGTCGGTGCCGAAACCTTTACGCGCGCATGCCAAGTCCAGCGTGAG
CTTGCCCACGTCGCCGGTCTGCATCACCGCAGCGTGAAGATCGCCGATCTTGTCATCGCCGCGGCGGCCGAACTTTCAGG
CACCATCGTGTGGCATTACGACGAGAACTATGACCGGGTCGCCGCCATCACCGGCCAACCTACGGAGTGGATCGTGCCGC
GCGGGACCCTTTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10406.91 Da
• Isoelectric Point: 4.6336

>AT156470 WP_000801914.1 NZ_CP053258:c3403764-3403486 [Escherichia coli]
MKPESKEAPINIRAKASQRDLIDMAANLVAKSRTDFMLDAACREAQDILLDQRLFIVDDEQYDAFLAALDAPITAERQAK
INALMNRKSPWE

Nucleotide

• Length: 279 bp

>AT156470 NZ_CP069065:2852343-2852600 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA