Detailed information of TA system TA156469

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 3352128..3352928
• Replicon: chromosome
• Accession: NZ_CP053258
• Organism: Escherichia coli strain GF4-3

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: HLY11_RS15530
• Protein ID: WP_171460219.1
• Coordinates: 3352401..3352928 (+)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: F4NNI1
• Locus tag: HLY11_RS15525
• Protein ID: WP_001277108.1
• Coordinates: 3352128..3352394 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HLY11_RS15505	3347787..3348455	+	669	WP_000617723.1	cell division ATP-binding protein FtsE	-
HLY11_RS15510	3348448..3349506	+	1059	WP_001042003.1	permease-like cell division protein FtsX	-
HLY11_RS15515	3349751..3350605	+	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
HLY11_RS15520	3350875..3351978	+	1104	WP_001021996.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
HLY11_RS15525	3352128..3352394	+	267	WP_001277108.1	DUF1778 domain-containing protein	Antitoxin
HLY11_RS15530	3352401..3352928	+	528	WP_171460219.1	GNAT family N-acetyltransferase	Toxin
HLY11_RS15535	3352925..3353308	-	384	WP_089622887.1	aspartate 1-decarboxylase autocleavage activator PanM	-
HLY11_RS15540	3353732..3354841	+	1110	WP_171460220.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
HLY11_RS15545	3354889..3355815	+	927	WP_001295097.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
HLY11_RS15550	3355812..3357089	+	1278	WP_000803782.1	branched chain amino acid ABC transporter permease LivM	-
HLY11_RS15555	3357086..3357853	+	768	WP_000082101.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19694.65 Da
• Isoelectric Point: 7.3502

>T156469 WP_171460219.1 NZ_CP053258:3352401-3352928 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRMAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Nucleotide

• Length: 528 bp

>T156469 NZ_CP069065:c2837767-2837348 [Mycobacterium tuberculosis]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9910.31 Da
• Isoelectric Point: 10.0863

>AT156469 WP_001277108.1 NZ_CP053258:3352128-3352394 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Nucleotide

• Length: 267 bp

>AT156469 NZ_CP069065:c2837988-2837764 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGTTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Structures

Antitoxin

• PDB: 6GTS
• PDB: 6AJN
• PDB: 6GTQ
• PDB: 6GTO
• PDB: 6GTR
• PDB: 6AJM
• AlphaFold DB: A0A829CN24