156468

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3329715..3330346 Replicon chromosome
Accession NZ_CP053258
Organism Escherichia coli strain GF4-3

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag HLY11_RS15395 Protein ID WP_001259385.1
Coordinates 3329715..3329990 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID Q31VE4
Locus tag HLY11_RS15400 Protein ID WP_000593554.1
Coordinates 3329987..3330346 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HLY11_RS15380 3324719..3325786 + 1068 WP_033870452.1 HlyD family efflux transporter periplasmic adaptor subunit -
HLY11_RS15385 3325783..3328518 + 2736 WP_171460208.1 ribosome-associated ATPase/putative transporter RbbA -
HLY11_RS15390 3328518..3329642 + 1125 WP_001216257.1 ABC transporter permease -
HLY11_RS15395 3329715..3329990 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
HLY11_RS15400 3329987..3330346 + 360 WP_000593554.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
HLY11_RS15405 3330466..3330867 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
HLY11_RS15410 3330873..3331679 - 807 WP_171460209.1 nickel import ATP-binding protein NikE -
HLY11_RS15415 3331676..3332440 - 765 WP_171460210.1 nickel import ATP-binding protein NikD -
HLY11_RS15420 3332440..3333273 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
HLY11_RS15425 3333270..3334214 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T156468 WP_001259385.1 NZ_CP053258:3329715-3329990 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T156468 NZ_CP069065:2791394-2791819 [Mycobacterium tuberculosis]
GTGGCGCTGCTCGACGTCAACGCATTGGTCGCGCTGGCGTGGGACTCACACATCCACCACGCCCGGATCCGCGAGTGGTT
TACCGCCAACGCCACGCTCGGCTGGGCGACTTGCCCGCTCACCGAAGCCGGCTTCGTGCGGGTGTCGACGAACCCAAAAG
TACTTCCCAGCGCGATCGGGATCGCAGACGCTCGACGGGTCCTCGTGGCACTACGCGCCGTGGGAGGCCACCGCTTCCTG
GCTGACGACGTATCGCTCGTCGATGACGATGTTCCGTTGATCGTCGGTTATCGCCAGGTGACCGACGCCCATCTGCTGAC
ACTCGCCCGCCGGCGCGGCGTCCGCCTGGTCACCTTCGACGCCGGTGTCTTCACCCTCGCCCAACAACGCCCCAAGACGC
CAGTGGAGCTGCTGACCATCCTCTAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13359.25 Da        Isoelectric Point: 4.8838

>AT156468 WP_000593554.1 NZ_CP053258:3329987-3330346 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT156468 NZ_CP069065:2791167-2791388 [Mycobacterium tuberculosis]
GTGCGAACGACACTTGACCTTGACGACGATGTGATCGCCGCGGCACGTGAACTTGCCTCCAGCCAGCGCCGCTCGCTCGG
CTCGGTGATTTCCGAACTCGCACGCCGTGGTCTCATGCCCGGACGCGTCGAGGCTGACGACGGGCTGCCGGTGATCCGCG
TTCCAGCCGGGACCCCGCCGATCACACCGGAGATGGTCCGTCGCGCGCTCGATGAGGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A6H2GM94

References